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The BD Cellismo™ Data Visualization Tool is a software tool for secondary analysis and visualization of single-cell multiomics data. It helps researchers turn millions of data points into actionable insights without writing a single line of code.
With the BD Cellismo™ Data Visualization Tool, you can easily visualize data from your samples across various parameters; subset cells into populations based on gene expression; create histograms, heatmaps, dot plots, volcano plots, violin plots, tSNE and UMAP plots; and customize and export plots as .PNG or .SVG files for collaboration and publication.
Watch the video to see how you can analyze your data using both primary analysis, with the BD Rhapsody™ Sequence Analysis Pipeline, and secondary analysis.
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Features
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Intuitive: Point and click to visualize your data as graphs and plots
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User Friendly: No cost of adoption; no coding required
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Multiomics Enabled: Explore single-cell RNA + protein datasets with ease
Generate the Same Data Visualization as Seurat Using the BD Cellismo™ Data Visualization Tool—Without Writing Any Code or Programming
With the BD Cellismo™ Data Visualization Tool (right) you’re able to generate the same data visualization as with Seurat (left), without having to write any code. The BD Cellismo™ Data Visualization Tool’s interface also lets you easily customize your figures (e.g., dot size, color, opacity) in a point and click fashion.

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Input Requirements:
- (Preferred) .Cellismo output file from the BD Rhapsody™ Sequence Analysis Pipeline. This file contains the expression data for all modalities (RNA and AbSeq) in addition to any cell annotations that are created by the pipeline (e.g., Sample Multiplexing Tag, Immune Cell Type).
- Alternative input formats:
- Market exchange (MEX)
- H5MU/H5AD

Release Notes
Latest Version: v1.0 | Release Date: March 2025
Initial release includes the following features:
- Load single-cell multiomic data from multiple formats, including BD Rhapsody™ Sequence Analysis Pipeline .Cellismo files with bundled metadata, matrix market MEX, Scanpy/Muon or H5MU/H5AD
- Manage multiple single-cell projects and jump between them with a few clicks
- Quality control with a visual on which cells are being removed
- Generate 7 different types of graphs: Dimensionality reduction scatter, Biaxial, Bioproduct correlation, Heatmap, Dot plot, Histogram and Violin.
- Extensive graph customization with style options
- Save images to a project gallery sidebar and recall them with ease
- Drag gallery images from the sidebar to external applications
- Create new cell annotations, by circling cells of interest, by running Leiden or K-means clustering, or by combining existing annotations
- Analyze differential expression between cell groups of interest. Filter genes of interest in a table and view on a volcano plot
- Generate new dimensionality reduction coordinates
- Supports whole transcriptome or targeted RNA, protein counts with AbSeq/CITE-seq and sample multiplexing
System requirements:
- Windows 10 or later (64-bit)
- MacOS 11 (Big Sur) or later
- Intel or Apple silicon chip
- 8 GB RAM minimum
- 16 GB RAM for 200,000 cells
- 64 GB RAM for 1M cells
"Cellismo is an excellent, lightweight bioinformatics tool for BD Rhapsody users, running on Windows and macOS. You can easily check sample quality, set analysis cutoffs, and view differentially expressed genes across clusters and samples. A key feature is using ribosomal RNA expression for deeper cell quality checks. For Abseq using BD’s antibodies, Cellismo lets you separate protein and mRNA expression with ease, making single-cell analysis straightforward and user-friendly."

"Cellismo is an excellent, lightweight bioinformatics tool for BD Rhapsody users, running on Windows and macOS. You can easily check sample quality, set analysis cutoffs, and view differentially expressed genes across clusters and samples. A key feature is using ribosomal RNA expression for deeper cell quality checks. For Abseq using BD’s antibodies, Cellismo lets you separate protein and mRNA expression with ease, making single-cell analysis straightforward and user-friendly."
"Cellismo made analysis and visualization of Rhapsody data simple and intuitive. Both people experienced with R and people who have never used R quickly performed tasks that typically take many lines of code. It simplified the preprocessing and graphing processes, facilitating deeper exploration of our data in far less time. The lasso tool and interactive DEG output made comparing populations of interest much easier as well. Cellismo is exactly the software we were looking for to rapidly analyze our single cell data with a minimal learning curve."

"Cellismo made analysis and visualization of Rhapsody data simple and intuitive. Both people experienced with R and people who have never used R quickly performed tasks that typically take many lines of code. It simplified the preprocessing and graphing processes, facilitating deeper exploration of our data in far less time. The lasso tool and interactive DEG output made comparing populations of interest much easier as well. Cellismo is exactly the software we were looking for to rapidly analyze our single cell data with a minimal learning curve."
"Cellismo is a program that is easy to use for both beginners and advanced users to perform all adjustments they need to get the most out of their data. I especially liked the numerous customization options for all types of plots. Additionally, the interactive design of the graphs makes data discussions so much easier, as all information for each individual data point being displayed is immediately at hand. Paired with the top-notch customer support that BD provides, I believe Cellismo will be a great tool for data analysis for any user."

"Cellismo is a program that is easy to use for both beginners and advanced users to perform all adjustments they need to get the most out of their data. I especially liked the numerous customization options for all types of plots. Additionally, the interactive design of the graphs makes data discussions so much easier, as all information for each individual data point being displayed is immediately at hand. Paired with the top-notch customer support that BD provides, I believe Cellismo will be a great tool for data analysis for any user."
"As researchers, we constantly seek tools that improve accuracy and save time, and Cellismo excels in both areas. Its intuitive interface and advanced analysis tools have simplified workflows that once required extensive manual effort. With Cellismo, we can visualize and interpret complex data with exceptional precision, allowing us to derive insights faster and more effectively. We are excited to continue exploring its capabilities as we push the boundaries of cellular research."

"As researchers, we constantly seek tools that improve accuracy and save time, and Cellismo excels in both areas. Its intuitive interface and advanced analysis tools have simplified workflows that once required extensive manual effort. With Cellismo, we can visualize and interpret complex data with exceptional precision, allowing us to derive insights faster and more effectively. We are excited to continue exploring its capabilities as we push the boundaries of cellular research."
"I had a great time working through the new Cellismo software. I was quickly able to navigate through the program to see what I wanted to see from our data. This program not only gets me to where I want to go independently, I also am not interrupting the workflow of my lab every time I get an itch with a new idea. I had a really fun time looking at our data with this program. I very much appreciate that BD has come up with this simple and user-friendly data visualization software package for people like me to use (and I will use this)."

"I had a great time working through the new Cellismo software. I was quickly able to navigate through the program to see what I wanted to see from our data. This program not only gets me to where I want to go independently, I also am not interrupting the workflow of my lab every time I get an itch with a new idea. I had a really fun time looking at our data with this program. I very much appreciate that BD has come up with this simple and user-friendly data visualization software package for people like me to use (and I will use this)."
"Cellismo is an excellent, lightweight bioinformatics tool for BD Rhapsody users, running on Windows and macOS. You can easily check sample quality, set analysis cutoffs, and view differentially expressed genes across clusters and samples. A key feature is using ribosomal RNA expression for deeper cell quality checks. For Abseq using BD’s antibodies, Cellismo lets you separate protein and mRNA expression with ease, making single-cell analysis straightforward and user-friendly."

"Cellismo is an excellent, lightweight bioinformatics tool for BD Rhapsody users, running on Windows and macOS. You can easily check sample quality, set analysis cutoffs, and view differentially expressed genes across clusters and samples. A key feature is using ribosomal RNA expression for deeper cell quality checks. For Abseq using BD’s antibodies, Cellismo lets you separate protein and mRNA expression with ease, making single-cell analysis straightforward and user-friendly."
"Cellismo made analysis and visualization of Rhapsody data simple and intuitive. Both people experienced with R and people who have never used R quickly performed tasks that typically take many lines of code. It simplified the preprocessing and graphing processes, facilitating deeper exploration of our data in far less time. The lasso tool and interactive DEG output made comparing populations of interest much easier as well. Cellismo is exactly the software we were looking for to rapidly analyze our single cell data with a minimal learning curve."

"Cellismo made analysis and visualization of Rhapsody data simple and intuitive. Both people experienced with R and people who have never used R quickly performed tasks that typically take many lines of code. It simplified the preprocessing and graphing processes, facilitating deeper exploration of our data in far less time. The lasso tool and interactive DEG output made comparing populations of interest much easier as well. Cellismo is exactly the software we were looking for to rapidly analyze our single cell data with a minimal learning curve."
"Cellismo is a program that is easy to use for both beginners and advanced users to perform all adjustments they need to get the most out of their data. I especially liked the numerous customization options for all types of plots. Additionally, the interactive design of the graphs makes data discussions so much easier, as all information for each individual data point being displayed is immediately at hand. Paired with the top-notch customer support that BD provides, I believe Cellismo will be a great tool for data analysis for any user."

"Cellismo is a program that is easy to use for both beginners and advanced users to perform all adjustments they need to get the most out of their data. I especially liked the numerous customization options for all types of plots. Additionally, the interactive design of the graphs makes data discussions so much easier, as all information for each individual data point being displayed is immediately at hand. Paired with the top-notch customer support that BD provides, I believe Cellismo will be a great tool for data analysis for any user."
"As researchers, we constantly seek tools that improve accuracy and save time, and Cellismo excels in both areas. Its intuitive interface and advanced analysis tools have simplified workflows that once required extensive manual effort. With Cellismo, we can visualize and interpret complex data with exceptional precision, allowing us to derive insights faster and more effectively. We are excited to continue exploring its capabilities as we push the boundaries of cellular research."

"As researchers, we constantly seek tools that improve accuracy and save time, and Cellismo excels in both areas. Its intuitive interface and advanced analysis tools have simplified workflows that once required extensive manual effort. With Cellismo, we can visualize and interpret complex data with exceptional precision, allowing us to derive insights faster and more effectively. We are excited to continue exploring its capabilities as we push the boundaries of cellular research."
"I had a great time working through the new Cellismo software. I was quickly able to navigate through the program to see what I wanted to see from our data. This program not only gets me to where I want to go independently, I also am not interrupting the workflow of my lab every time I get an itch with a new idea. I had a really fun time looking at our data with this program. I very much appreciate that BD has come up with this simple and user-friendly data visualization software package for people like me to use (and I will use this)."

"I had a great time working through the new Cellismo software. I was quickly able to navigate through the program to see what I wanted to see from our data. This program not only gets me to where I want to go independently, I also am not interrupting the workflow of my lab every time I get an itch with a new idea. I had a really fun time looking at our data with this program. I very much appreciate that BD has come up with this simple and user-friendly data visualization software package for people like me to use (and I will use this)."
"Cellismo is an excellent, lightweight bioinformatics tool for BD Rhapsody users, running on Windows and macOS. You can easily check sample quality, set analysis cutoffs, and view differentially expressed genes across clusters and samples. A key feature is using ribosomal RNA expression for deeper cell quality checks. For Abseq using BD’s antibodies, Cellismo lets you separate protein and mRNA expression with ease, making single-cell analysis straightforward and user-friendly."

"Cellismo is an excellent, lightweight bioinformatics tool for BD Rhapsody users, running on Windows and macOS. You can easily check sample quality, set analysis cutoffs, and view differentially expressed genes across clusters and samples. A key feature is using ribosomal RNA expression for deeper cell quality checks. For Abseq using BD’s antibodies, Cellismo lets you separate protein and mRNA expression with ease, making single-cell analysis straightforward and user-friendly."


Free, but Powerful–Try it Out for Yourself
Download the free BD Cellismo™ Data Visualization Tool today. After you provide your information and click Submit, download will begin automatically. We will use your information to keep you updated on latest developments, updates and bug fixes as they become available.
Windows Users:
Once the file has downloaded, double-click the .exe file. You will see a simple animation as it installs. After the install is complete, the program will open automatically. A shortcut will be placed on your desktop.
Mac Users:
There are two different install packages depending on your Chip architecture—Mac (Intel) or Mac (Apple M). To check your chip architecture, press the Apple menu on the top left. Select About This Mac; where it says Chip, it should say either Intel or Apple. In the form below, pick the option that applies to your system. Once the file has downloaded, double-click the .zip file to extract Cellismo.app. Double-click Cellismo.app to open it. The app can be moved to the folder of your choice (like the user's Applications directory).
Once installed, start new projects on the Home page under the Start New Project tab. Drag-and-drop data files onto the page and click Start Analysis.

Download the Free BD Cellismo™ Tool
After you provide your information and click the Submit button, download will begin automatically.
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