Single-Cell Plant Technologies Webinar

Novel approaches to single-cell plant research: From Real-Time Imaging cell sorting to single-nuclei transcriptomics

The introduction of real-time imaging in flow cytometry and the recent advancement in single-cell multiomics have revolutionized the world of cellular and molecular biology by adding new dimensions to single-cell data analysis. Unsurprisingly, these technologies are also gaining traction in plant molecular and developmental biology to reveal the transcriptional changes across cell types in a specific tissue or organ, upon treatments, in response to biotic and abiotic stresses, or between genotypes.

In this webinar, Gert Van Isterdael will present an overview of the plant applications they have been developing using the BD FACSDiscover^™S8 Cell Sorter in collaboration with researchers from the VIB-UGent Center for Plant Systems Biology. The use of BD CellView^™ Image Technology applied to protoplasts allows scientists to make faster and more confident sorting decisions, thereby improving the quality of sort outcomes and its connected downstream processes.

In the second part of the webinar, Dr. Carolin Grones will address the challenges of single-cell transcriptomics applied to plant tissues while proposing a novel single-nuclei approach to deliver more robust and high-quality data, thanks to the BD Rhapsody^™Single-Cell Analysis System.

Learn How:

Plant molecular and developmental biology can benefit from new technologies that allow real-time imaging and single-cell multiomics of plant cells.
The BD FACSDiscover^™ S8 Cell Sorter can sort protoplasts based on their image features and improve the quality of downstream processes.
The BD Rhapsody^™ Single-Cell Analysis System can perform single-nuclei transcriptomics on plant tissues and deliver more reliable and comprehensive data.

Speakers

Carolin Grones, PhD, VIB-UGent Center for Plant Systems Biology and Wageningen University

Carolin Grones (PhD) is working as single cell application specialist at the VIB-UGent Center for Plant Systems Biology (Gent, Belgium) and at Wageningen University (Wageningen, The Netherlands). She obtained her PhD at the Max Planck Institute for Plant Breeding Research in Cologne (Germany) studying hormone signaling networks during plant-microbe interactions. She continued her research in plant hormone-mediated transcriptional regulation of developmental processes during her postdoc at Umea University (Sweden) and the VIB-UGent Center for Plant Systems Biology. A major focus of her research in the last years was to evaluate single cell technologies for their compatibility with plant samples. Together with the VIB Flow Core and the VIB Single Cell Core she continuously optimizes protocols for cell and nuclei isolation in crops and many other plant species.

Gert Van Isterdael, BSc, head of VIB Flow Core Gent

Gert Van Isterdael is head of the VIB Flow Core Ghent. After obtaining his Bachelor of Science, he was subsequently a member of the De Jaeger lab (2003-2007) and the Beeckman lab (2007-2013) at the VIB Center of Plant Systems Biology (PSB). During his time at PSB, he became the cell sorter operator for the department and his passion for flow cytometry started. Early 2013, he joined the VIB Center for Inflammation Research (IRC) in the Lambrecht and Hammad lab. He became responsible for all flow cytometers and cell sorters located at IRC and was strongly involved in the conception of the IRC Flow Core facility, which matured in the VIB Flow Core Ghent. His primary role is to provide flow cytometry expertise to the users of the core facility. Under his leadership the VIB Flow Core has become a top academic core facility within Europe and the core is actively involved in benchmarking and testing new technologies. Gert Van Isterdael is an active member of ISAC, the International Society for Advancement of Cytometry and has received an ISAC SRL Emerging leader award (2016-2020) and the RMS Award for Flow Cytometry 2021.

Watch the Single-Cell Plant Technologies Webinar On-Demand

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