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BD Horizon RealYellow™ and RealBlue™ Reagents

Deliver reduced spillover and optimize resolution when used with other fluorochromes. Try out our latest BD Horizon RealBlue™ 670 (RB670) Reagents.

Still Using PerCP and PE Tandem Dyes?
Cleaner Alternatives Are Here.

The family of BD Horizon RealYellow™ and BD Horizon RealBlue™ Reagents is bright, clean and laser specific to help streamline the path to your scientific discoveries.

 

Driven by next-generation BD® dye technology and AI-guided fluorochrome selection to optimize spectral positioning, our new family of reagents is specially engineered to deliver reduced spillover to optimize resolution when used with other fluorochromes—helping to expand your experiments on both conventional and spectral flow cytometers.

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    When building assays, we are presented with hundreds of fluorochrome options and their different properties can make selection feel overwhelming. Check out our Fluorochrome Performance Chart with a handy cheat-sheet to help you prioritise clean fluorochromes and simplify your panel design.

    Interview with Jochen Lamote, Head of VIB Flow Core



    BD Biosciences sat down with Jochen Lamote to discuss his experience as a beta tester of flow cytometry reagents – including how BD Horizon RealBlue minimizes spillover and simplifies panel design.


    Watch the interview to discover why the team at Vlaams Institute for Biotechnology (VIB) Flow Core would recommend BD Horizon RealBlueto their colleagues.

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      Still Using PerCP- and PE-Based Tandem Dyes? Time for an Upgrade.

      FormatSpectralConventionalRelative BrightnessSpillover
      (1-low, 4-high)
      Alternative To
      Blue Laser Line
      RB545  1(Unique position)
      RB613 2PE-CF594, PE-Dazzle™ 594, BB630-P2 or StarBright™ Blue 615
      RB670 2PE-Cy5, BB660-P2 or StarBright™ Blue 675
      RB705 2PerCP-Cy5.5, BB700 or StarBright™ Blue 700
      RB744 1BB755-P
      RB780 1PE-Cy7 or BB790-P
      Yellow-Green Laser Line
      RY586 1PE
      RY610 1PE-CF594 or PE-Dazzle™ 594
      RY703 2PE-Cy5.5 or StarBright™ Yellow 720
      RY775 2PE-Cy7 or StarBright™ Yellow 800
      Note: PE and PE tandem dyes are excited by both the blue and yellow-green lasers. Be sure to check your instrument configuration

       

       

      RB545
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      BD Horizon RealBlue™ 545 (RB545) Reagents

      RB545 reagents are innovative laser-specific fluorochromes excited primarily by the 488-nm blue laser that offer:

       

      • Minimal cross-laser excitation off the 561-nm yellow-green laser
      • Moderate brightness fluorochrome to support the detection of high-expression surface and intracellular markers

       

      Download the BD Horizon RealBlue™ 545 Brochure>

       
      RB545 vials
      performance1

      RB545 reagents can be used on spectral cytometers to increase the number of experimental parameters.

      Performance

      BD Horizon™ RB545 Reagents offer minimal cross-laser excitation off the yellow-green laser

      RB545 Normalized Emission Filter Parameters
      apps1
      RB545 Spread Plots
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      Applications

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      BD Horizon RealBlue™ 545 Reagents are compatible with a broad range of fixation and permeabilization systems

       

      BuffersResults
      BD FACS™ Lysing Solution and BD Pharm Lyse™ Lysing BufferCompatible
      CellBlox™ Blocking BufferCompatible
      BD Cytofix™ Fixation BufferStable at least 24 hours
      1% PFAStable at least 24 hours
      BD Cytofix/Cytoperm™ Fixation and Permeabilization SolutionCompatible with antibody staining before and after fixation
      BD FACS™ Permeabilizing Solution IICompatible with antibody staining before and after fixation
      BD Phosflow™ Perm Buffer IIICompatible with antibody staining before and after fixation
      EDTA and HeparinCompatible
      BD Horizon™ Brilliant Stain Buffer (BSB)Compatible
       
      RB613
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      RB670
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      RB705
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      RB744
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      RB780
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      RY586
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      RY610
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      RY703
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      RY775
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      "The RY775 CD8 antibody worked very well and slotted easily into my panel."

      Fritz Lea

      "The RY775 CD8 antibody worked very well and slotted easily into my panel."

      Lea Fritz
      PhD student
      Institute for Immunology, Hannover Medical School
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      Terms and Conditions

      Offer valid from January 1st 2025 – March 31st 2025. Cannot be combined with any other offer. Valid in participating countries only. Valid once per user. Offer valid on BD Horizon RealBlue™ 670 (RB670) Reagents 25 test/ug size only. There are a limited number of samples available, and BD retains the right to cancel this offer at any time.

       

      For Research Use Only. Not for use in diagnostic or therapeutic procedures. BD, the BD Logo, BD Horizon RealBlue and BD Horizon RealYellow are trademarks of Becton, Dickinson and Company or its affiliates. All other trademarks are the property of their respective owners. © 2024 BD. All rights reserved. BD-132104 (v1.0) 0724 CF is a trademark of Biotium, Inc. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.

       

      BD FACSLyric Flow Cytometer with the BD FACSuite™ Clinical and BD FACSuite applications is an in vitro diagnostic medical device bearing a CE mark.