BD OptiBuild™ BV750 Rat Anti-Mouse TCR Vβ8.1, 8.2
Clone KJ16-133 (also known as KJ16; KJ-16-133; KJ-16)
(RUO)
Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Product Notices
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- BD Horizon Brilliant™ Violet 750 is covered by one or more of the following US patents: 8,158,444; 8,802,450; 8,575,303; 8,455,613; 8,227,187; 8,841,072; 8,110,673.
Companion Products
The KJ16-133 monoclonal antibody specifically recognizes Mouse T cell receptors (TCR) containing β-chains with TCR Vβ8.1 or TCR Vβ8.2 regions that belong to TCR Vβ8 family which also includes TCR Vβ8.3. TCR Vβ8.1 and TCR Vβ8.2 (TCR Vβ8.1, 8.2) are alternatively expressed by subpopulations of mouse thymocytes and peripheral CD4+ and CD8+ T cells in most mouse strains including BALB/c, C57BL6, B10.A, and AKR. The Tcrb-V8 subfamily gene loci are deleted in mice having the a (eg, C57BR, C57L, SJL, SWR) or c (eg, RIII) MHC haplotype. Mouse mammary tumor viruses (MMTV) exist in two forms as either germline integrants in the mouse genome (Mtv) or as infectious virus particles that can affect the size of TCR Vβ 8+ T cell subpopulations. TCR Vβ 8.1+ T cells are clonally eliminated in mice expressing superantigen encoded by the Mtv-7 provirus (eg, AKR, CBA/J, C58, DBA/2), and activation or elimination of TCR Vβ 8.1+ T cells by this determinant is partially dependent upon presentation by I-E. Mtv-43, Mtv-44, and/or exogenous MMTV-SW superantigens also cause incomplete elimination of TCR Vβ 8.1+ T cells. Likewise, staphylococcal enterotoxin B (SEB), in association with antigen presenting cells expressing I-A and/or I-E, stimulates TCR Vβ 8.1+ T cells and selectively eliminates those T cells in vivo. In addition to expression on conventional T lymphocytes, Vβ8.2 is the predominant TCR β chain variable region expressed on NK-T cells.
Development References (8)
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Behlke MA, Chou HS, Huppi K, Loh DY. Murine T-cell receptor mutants with deletions of beta-chain variable region genes. Proc Natl Acad Sci U S A. 1986; 83(3):767-771. (Biology). View Reference
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Braciale TJ, Henkel TJ, Lukacher A, Braciale VL. Fine specificity and antigen receptor expression among influenza virus-specific cytolytic T lymphocyte clones.. J Immunol. 1986; 137(3):995-1002. (Clone-specific: Flow cytometry). View Reference
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Farr AG, Anderson SK, Marrack P, Kappler J. Expression of antigen-specific, major histocompatibility complex-restricted receptors by cortical and medullary thymocytes in situ.. Cell. 1985; 43(2 Pt 1):543-50. (Clone-specific: Electron microscopy, Immunohistochemistry). View Reference
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Haqqi TM, Banerjee S, Anderson GD, David CS. RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes. J Exp Med. 1989; 169(6):1903-1909. (Biology). View Reference
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Haskins K, Hannum C, White J, et al. The antigen-specific, major histocompatibility complex-restricted receptor on T cells. VI. An antibody to a receptor allotype.. J Exp Med. 1984; 160(2):452-71. (Immunogen: Blocking, ELISA, Flow cytometry, Immunoprecipitation, Inhibition, Radioimmunoassay). View Reference
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Luther S, Shakhov AN, Xenarios I, Haga S, Imai S, Acha-Orbea H. New infectious mammary tumor virus superantigen with V beta-specificity identical to staphylococcal enterotoxin B (SEB).. Eur J Immunol. 1994; 24(8):1757-64. (Biology). View Reference
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Roehm N, Herron L, Cambier J, et al. The major histocompatibility complex-restricted antigen receptor on T cells: distribution on thymus and peripheral T cells.. Cell. 1984; 38(2):577-84. (Clone-specific: Flow cytometry). View Reference
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White J, Herman A, Pullen AM, Kubo R, Kappler JW, Marrack P. The V beta-specific superantigen staphylococcal enterotoxin B: stimulation of mature T cells and clonal deletion in neonatal mice. Cell. 1989; 56(1):27-35. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.