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      BV421 Mouse Anti-Human TCR Vα7.2
      BV421 Mouse Anti-Human TCR Vα7.2
      Multiparameter flow cytometric analysis using BD OptiBuild™ BV421 Mouse Anti-Human TCR Vα7.2 antibody (Cat. No. 749494) on human peripheral blood PE CD3 lymphocytes. Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.
      BV421 Mouse Anti-Human TCR Vα7.2
      Multiparameter flow cytometric analysis using BD OptiBuild™ BV421 Mouse Anti-Human TCR Vα7.2 antibody (Cat. No. 749494) on human peripheral blood PE CD3 lymphocytes. Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.
      Product Details
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      BD OptiBuild™
      TRAV7; TCR V alpha 7.2; T cell receptor alpha variable 7.2; TCRAV7.2
      Human (Tested in Development)
      Mouse IgG1, κ
      Human Va7.2-expressing T cell clones
      Flow cytometry (Qualified)
      0.2 mg/ml
      AB_2873862
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.
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      Recommended Assay Procedures

      For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

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      Product Notices

      1. This antibody was developed for use in flow cytometry.
      2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      3. Researchers should determine the optimal concentration of this reagent for their individual applications.
      4. An isotype control should be used at the same concentration as the antibody of interest.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
      9. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
      10. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
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      749494 Rev. 3
      Antibody Details
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      OF-5A12

      The OF-5A12 monoclonal antibody specifically recognizes the human variable alpha 7.2 region of the T cell receptor for antigen (TCR Vα7.2) that is joined with the Jα33 segment to form an invariant TCR which is characteristically expressed by mucosal-associated invariant T cells (MAIT cells). MAIT cell recognition of antigen is restricted by MHC-related molecule 1 (MR1), a nonpolymorphic Class Ib Major Histocompatibility Complex (MHC) molecule, that associates with β2-microglobulin. MAIT cells are found in human peripheral blood, mesenteric lymph nodes, liver, and mucosa associated with the lungs and intestines. MAIT cells play a role in detecting and fighting off microbial infections. The specificity of OF-5A12 for the TCR Vα7.2 was confirmed by its ability to block the binding of another human TCR Vα7.2-specific antibody, 3C10, to MAIT cells.

      The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

      749494 Rev. 3
      Format Details
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      BV421
      The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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      BV421
      Violet 405 nm
      407 nm
      423 nm
      749494 Rev.3
      Citations & References
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      View product citations for antibody "749494" on CiteAb

      Development References (3)

      1. Godfrey DI, Uldrich AP, McCluskey J, Rossjohn J, Moody DB. The burgeoning family of unconventional T cells. Nat Immunol. 2015; 16(11):1114-1123. (Biology). View Reference
      2. Gold MC, Cerri S, Smyk-Pearson S, et al. Human mucosal associated invariant T cells detect bacterially infected cells. PLoS Biol. 2010; 8(6):e1000407. (Clone-specific). View Reference
      3. Gold MC, Eid T, Smyk-Pearson S, et al. Human thymic MR1-restricted MAIT cells are innate pathogen-reactive effectors that adapt following thymic egress. Mucosal Immunol. 2013; 6(1):35-44. (Immunogen). View Reference
      749494 Rev. 3

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.