Preparation And Storage
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Product Notices
- This antibody was developed for use in flow cytometry.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
Companion Products
The 89412 monoclonal antibody specifically recognizes the Interleukin-1 Receptor Accessory Protein (IL-1RAcP) which is also known as Interleukin-1 Receptor 3 (IL-1R3). IL-1RAcP (IL-1R3) is a type I transmembrane glycoprotein that is encoded by IL1RAP (interleukin 1 receptor accessory protein) which belongs to the immunoglobulin gene superfamily (IgSF). IL-1RAcP (IL-1R3) is comprised of an extracellular domain with three IgC-like domains, followed by a transmembrane region, and a cytoplasmic region with a Toll/IL-1R (TIR) domain. IL-1RAcP (IL-1R3) is widely expressed at low levels. This coreceptor associates with other ligand-binding chains, IL-1R1/CD121a (for ligands IL-1a, IL-1b, or IL-38), IL-1R4 (for IL-33), or IL-1R6 (for IL-36a, b, g, or IL-38) to form functional signaling receptor complexes. The cellular response to these ligands can be modulated through a non-signaling association of IL-1RAcP (IL-1R3) with the membrane IL1R2/CD121b decoy receptor.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
Development References (5)
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Bianchetti L, Marini MA, Isgrò M, Bellini A, Schmidt M, Mattoli S. IL-33 promotes the migration and proliferation of circulating fibrocytes from patients with allergen-exacerbated asthma.. Biochem Biophys Res Commun. 2012; 426(1):116-21. (Clone-specific: Flow cytometry). View Reference
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Boraschi D, Italiani P, Weil S, Martin MU. The family of the interleukin-1 receptors. Immunol Rev. 2018; 281(1):197-232. (Biology). View Reference
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Brikos C, Wait R, Begum S, O'Neill LA, Saklatvala J. Mass spectrometric analysis of the endogenous type I interleukin-1 (IL-1) receptor signaling complex formed after IL-1 binding identifies IL-1RAcP, MyD88, and IRAK-4 as the stable components.. Mol Cell Proteomics. 2007; 6(9):1551-9. (Biology). View Reference
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Chackerian AA, Oldham ER, Murphy EE, Schmitz J, Pflanz S, Kastelein RA. IL-1 receptor accessory protein and ST2 comprise the IL-33 receptor complex.. J Immunol. 2007; 179(4):2551-5. (Biology). View Reference
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Greenfeder SA, Nunes P, Kwee L, Labow M, Chizzonite RA, Ju G. Molecular cloning and characterization of a second subunit of the interleukin 1 receptor complex.. J Biol Chem. 1995; 270(23):13757-65. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.