Preparation And Storage
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Product Notices
- This antibody was developed for use in flow cytometry.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Violet 480 is covered by one or more of the following US patents: 8,575,303; 8,354,239.
Companion Products
The WTH2 monoclonal antibody specifically binds to rat CD1d and crossreacts with mouse CD1d. CD1d is a type I transmembrane glycoprotein that is noncovalently associated with β2-microglobulin. CD1d is expressed on various cell types including subsets of thymocytes, T cells, B cells, monocytes, macrophages, dendritic cells, endothelial cells and epithelial cells. Although structurally similar to MHC Class I antigens, CD1d molecules are rather non-polymorphic and serve to present non-peptide antigens such as endogenous or microbial glycolipids to T lymphocytes (NKT cells). The WTH1 monoclonal antibody reportedly binds to a non-overlapping epitope on rat CD1d and also crossreacts with mouse CD1d when compared with WTH2. Both WTH1 and WTH2 reportedly interfere with antigen recognition by CD1d-restricted T cells.
The antibody was conjugated to BD Horizon™ BV480 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 436-nm and Em Max at 478-nm, BD Horizon BV480 can be excited by the violet laser and detected in the BD Horizon BV510 (525/40-nm) filter set. BV480 has less spillover into the BV605 detector and, in general, is brighter than BV510.
Development References (3)
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Ichimiya S, Kikuchi K, Matsuura A. Structural analysis of the rat homologue of CD1. Evidence for evolutionary conservation of the CD1D class and widespread transcription by rat cells. J Immunol. 1994; 153(3):1112-1123. (Biology). View Reference
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Katabami S, Matsuura A, Chen HZ, Imai K, Kikuchi K. Structural organization of rat CD1 typifies evolutionarily conserved CD1D class genes. Immunogenetics. 1998; 48(1):22-31. (Biology). View Reference
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Monzon-Casanova E, Steiniger B, Schweigle S, et al. CD1d expression in paneth cells and rat exocrine pancreas revealed by novel monoclonal antibodies which differentially affect NKT cell activation. PLoS ONE. 2010; 5(9):e13089. (Immunogen: Blocking, ELISA, Flow cytometry, Functional assay, Immunohistochemistry, Immunoprecipitation, Inhibition, Western blot). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.