Preparation And Storage
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Product Notices
- This antibody was developed for use in flow cytometry.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
Companion Products
The UP-D1 monoclonal antibody specifically binds to Immune receptor expressed on myeloid cells 1 (IREM-1). IREM-1 is encoded by CD300LF. It is also known as CD300 antigen-like family member F (CD300f), CMRF35-like molecule 1 (CLM-1/CLM1), NK inhibitory receptor (NKIR), or Immunoglobulin superfamily member 13 (IgSF13). IREM-1/CD300f is expressed on monocytes, dendritic cells, granulocytes, and mast cells. IREM-1 is a single-pass type I transmembrane glycoprotein that belongs to the CMRF family of the Ig gene superfamily. The long intracellular region of IREM-1 contains two classical immunoreceptor tyrosine-based inhibitory motifs (ITIM). Ligand-bound IREM-1 can transduce negative regulatory signals intracellularly by recruiting SHP1 or SHIP phosphatases that in turn dephosphorylate tyrosine residues within activating cell signaling molecules. CD300f may thus play a role in regulating inflammatory responses involving myeloid cells.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
Development References (6)
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Aguilar H, Alvarez-Errico D, García-Montero AC, Orfao A, Sayós J, López-Botet M. Molecular characterization of a novel immune receptor restricted to the monocytic lineage. J Immunol. 2004; 173(11):6703-6711. (Biology). View Reference
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Alvarez-Errico D, Aguilar H, Kitzig F, Brckalo T, Sayós J, López-Botet M. IREM-1 is a novel inhibitory receptor expressed by myeloid cells. Eur J Immunol. 2004; 34(12):3690-3701. (Immunogen: ELISA, Flow cytometry, Functional assay, Immunoprecipitation, Inhibition). View Reference
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Alvarez-Errico D, Sayós J, López-Botet M. The IREM-1 (CD300f) inhibitory receptor associates with the p85alpha subunit of phosphoinositide 3-kinase. J Immunol. 2007; 178(2):808-816. (Clone-specific: Flow cytometry, Functional assay, Inhibition). View Reference
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Borrego F. The CD300 molecules: an emerging family of regulators of the immune system. Blood. 2013; 121(11):1951-1960. (Biology). View Reference
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Lee SM, Suk K, Lee WH. Synthetic peptides containing ITIM-like sequences of IREM-1 (CD300F) differentially regulate MyD88 and TRIF-mediated TLR signalling through activation of SHP and/or PI3K. Clin Exp Immunol. 2012; 167(3):438-446. (Biology). View Reference
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Sui L, Li N, Liu Q, et al. IgSF13, a novel human inhibitory receptor of the immunoglobulin superfamily, is preferentially expressed in dendritic cells and monocytes. Biochem Biophys Res Commun. 2004; 319(3):920-928. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.