Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
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CD100 was originally described as a 150 kDa surface T-cell antigen induced after T-cell activation. Human CD100 exhibits widespread gene expression but is most prominent in peripheral lymphocytes, thymus, skeletal muscle, and brain. The full CD100 protein is 862 amino acids with homolgy to the semaphorin family of proteins at its extracellular domain followed by a short Ig-like domain. The short cytoplasmic domain contains a putative tyrosine phosphorylation site which may be the site for association with intracellular kinases. Cells that overexpress CD100 induce a rapid aggregation of B cells and increase the CD90L-B cell aggregation. Because semaphorins are axon-guiding molecules for the nervous system, it has been proposed that CD100 may have a guidance role in the immune system.
Development References (1)
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Hall KT, Boumsell L, Schultze JL, et al. Human CD100, a novel leukocyte semaphorin that promotes B-cell aggregation and differentiation. Proc Natl Acad Sci U S A. 1996; 93(21):11780-11785. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.