Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
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Annexin VII, also known as synexin, is a member of the Annexin family characterized by Ca2+ dependent phospholipid binding. Annexins have a four-fold internal repeat of about 70 amino acids that contains the Ca2+-binding sites. They also have a regulatory NH2-terminal region of 30-40 amino acids. Annexin VII mRNA has been detected in many tissues and is most abundant in brain, heart, skeletal muscle, and lung. Annexin VII mRNA exhibits a tissue dependent polymorphism due to alternative splicing which results in two different isoforms of 47 kDa and 51 kDa. In vitro, Annexin VII aggregates chromaffin granules and enhances membrane fusion in a Ca2+ and GTP-dependent manner. Although the physiological role of Annexin VII is not completely understood, it is believed to influence and regulate Ca2+ dependent events such as secretion at the plasma membrane.
Development References (5)
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Furge LL, Chen K, Cohen S. Annexin VII and annexin XI are tyrosine phosphorylated in peroxovanadate-treated dogs and in platelet-derived growth factor-treated rat vascular smooth muscle cells. J Biol Chem. 1999; 274(47):33504-33509. (Biology: Western blot). View Reference
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Magendzo K, Shirvan A, Cultraro C, Srivastava M, Pollard HB, Burns AL. Alternative splicing of human synexin mRNA in brain, cardiac, and skeletal muscle alters the unique N-terminal domain. J Biol Chem. 1991; 266(5):3228-3232. (Biology). View Reference
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Salzer U, Hinterdorfer P, Hunger U, Borken C, Prohaska R. Ca(++)-dependent vesicle release from erythrocytes involves stomatin-specific lipid rafts, synexin (annexin VII), and sorcin. Blood. 2002; 99(7):2569-2577. (Biology: Immunohistochemistry, Western blot). View Reference
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Selbert S, Fischer P, Pongratz D, Stewart M, Noegel AA. Expression and localization of annexin VII (synexin) in muscle cells. J Cell Sci. 1995; 108(Pt 1):85-95. (Biology). View Reference
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Srivastava M, Bubendorf L, Srikantan V. ANX7, a candidate tumor suppressor gene for prostate cancer. Proc Natl Acad Sci U S A. 2001; 98(8):4575-4580. (Biology: Immunohistochemistry). View Reference
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