Alexa Fluor™ 647 Mouse Anti-Human VSTM1 (SIRL-1)

BD Pharmingen™ Alexa Fluor™ 647 Mouse Anti-Human VSTM1 (SIRL-1)

Name: Alexa Fluor™ 647 Mouse Anti-Human VSTM1 (SIRL-1)
Brand: BD Pharmingen™
SKU: 568957

Clone 1A5/SIRL-1 (also known as 1A5)

(RUO)

Multiparameter flow cytometric analysis of VSTM1 (SIRL-1) expression on different Human peripheral blood leucocyte populations. Human whole blood was stained with either Alexa Fluor™ 647 Mouse IgG1, κ Isotype Control (Cat. No. 565571; Left Plot) or Alexa Fluor™ 647 Mouse Anti-Human VSTM1 (SIRL-1) antibody (Cat. No. 568957/568958; Right Plot). After staining, the erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The bivariate pseudocolor density plot showing the correlated expression of VSTM1 (SIRL-1) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.

Spectrum Viewer

Protocol Library

Scientific Resources

Product Details

Brand: BD Pharmingen™

Alternative Name: VSTM1; SIRL1; SIRL-1

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Immunogen: Purified SIRL-1-hIg Fusion Protein

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl/test

Entrez Gene ID: 284415

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
An isotype control should be used at the same concentration as the antibody of interest.
This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Alexa Fluor™ is a trademark of Life Technologies Corporation.
For U.S. patents that may apply, see bd.com/patents.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Alexa Fluor® 647 Mouse IgG1 κ Isotype Control RUO

Size 50 µg Cat No. 565571

Lysing Buffer RUO

Size 100 mL Cat No. 555899

Lysing Solution 10X Concentrate IVD

Size 100 mL Cat No. 349202

568957 Rev. 4

Antibody Details

1A5/SIRL-1

The 1A5 (1A5/SIRL-1) monoclonal antibody specifically recognizes human V-set and transmembrane domain-containing protein 1 (VSTM1) which is also known as Signal Inhibitory Receptor on Leucocytes-1 (SIRL-1). VSTM1 (SIRL-1) is a single-pass type I transmembrane glycoprotein that contains an IgV-like domain in its extracellular region which is followed by a transmembrane segment and an intracellular region with two immunoreceptor tyrosine-based inhibitory motifs (ITIMs). This molecule is encoded by VSTM1 (V-set and transmembrane domain containing 1) which belongs to the transmembrane receptor Ig superfamily of immune inhibitory receptors. VSTM1 (SIRL-1) is variably expressed on myeloid cells including neutrophils, eosinophils, and monocytes. SIRL-1 recognizes phenol-soluble modulins of Staphylococcus as well as danger- or damage-associated molecular patterns (DAMPs) including S100 proteins and the antimicrobial peptide, LL-37. VSTM1 (SIRL-1) regulates innate effector functions including Fc Receptor-induced production of reactive oxygen species (ROS) and formation of neutrophil extracellular traps (NET) released by human neutrophils in response to certain bacterial and inflammatory danger signals. Although NETs serve to trap and kill extracellular microbes, VSTM1 (SIRL-1)-mediated regulation of NETs expression helps avoid excessive tissue damage caused by inflammation and potential induction or exacerbation of autoimmune disease. VSTM1 (SIRL-1) function does not impair neutrophil phagocytosis and killing of intracellular bacteria. VSTM1 (SIRL-1) expression is downregulated in response to microbial stimuli which allows microbial killing and clearance of pathogens.

568957 Rev. 4

Format Details

Alexa Fluor™ 647

Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: Alexa Fluor™ 647

Excitation Source: Red 627-640 nm

Excitation Max: 653 nm

Emission Max: 669 nm

568957 Rev.4

Citations & References

View product citations for antibody "568957" on CiteAb

Development References (4)

Rumpret M, von Richthofen HJ, Peperzak V, Meyaard L. Inhibitory pattern recognition receptors.. J Exp Med. 2022; 219(1):e20211463. (Biology). View Reference
Steevels TA, Lebbink RJ, Westerlaken GH, Coffer PJ, Meyaard L. Signal inhibitory receptor on leukocytes-1 is a novel functional inhibitory immune receptor expressed on human phagocytes.. J Immunol. 2010; 184(9):4741-8. (Immunogen: Flow cytometry, Functional assay, Immunoprecipitation, Western blot). View Reference
Steevels TA, van Avondt K, Westerlaken GH, et al. Signal inhibitory receptor on leukocytes-1 (SIRL-1) negatively regulates the oxidative burst in human phagocytes.. Eur J Immunol. 2013; 43(5):1297-308. (Clone-specific: Flow cytometry). View Reference
Van Avondt K, van der Linden M, Naccache PH, Egan DA, Meyaard L. Signal Inhibitory Receptor on Leukocytes-1 Limits the Formation of Neutrophil Extracellular Traps, but Preserves Intracellular Bacterial Killing.. J Immunol. 2016; 196(9):3686-94. (Clone-specific: Functional assay). View Reference

View All (4)

568957 Rev. 4

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.