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Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Companion Products
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The ER-TR9 monoclonal antibody specifically recognizes mouse CD209 antigen-like protein B (CD209b) which is also known as Mouse SIGN-related 1 (mSIGNR1 or SIGNR1). CD209b is a mouse homolog of human CD209 (DC-SIGN) and is also referred to as DC-SIGN-related protein 1 (DC-SIGNR1). CD209b is an ~37 kDa type II transmembrane glycoprotein that is encoded by Cd209b (CD209b antigen) which belongs to the C-type lectin family. It contains a single C-terminal C-type lectin domain and is involved in the recognition of microbial polysaccharides. CD209b is expressed on macrophages residing in the splenic marginal zone, the medullary and trabecular sinuses of lymph nodes, and peritoneal cavity. CD209b is involved in the clearance and innate immune responses to microbial molecules including polysaccharides and lipopolysaccharides (LPS) and the uptake of encapsulated microbes. It may also affect leucocyte migration through its adhesive interaction with CD54 (ICAM-2).
Development References (7)
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Dijkstra CD, Van Vliet E, Döpp EA, van der Lelij AA, Kraal G. Marginal zone macrophages identified by a monoclonal antibody: characterization of immuno- and enzyme-histochemical properties and functional capacities.. Immunology. 1985; 55(1):23-30. (Clone-specific: Immunohistochemistry). View Reference
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Geijtenbeek TB, Groot PC, Nolte MA, et al. Marginal zone macrophages express a murine homologue of DC-SIGN that captures blood-borne antigens in vivo.. Blood. 2002; 100(8):2908-16. (Clone-specific: ELISA, Flow cytometry). View Reference
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Karlsson MC, Guinamard R, Bolland S, Sankala M, Steinman RM, Ravetch JV. Macrophages control the retention and trafficking of B lymphocytes in the splenic marginal zone.. J Exp Med. 2003; 198(2):333-40. (Clone-specific: Immunohistochemistry). View Reference
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Leenen PJ, de Bruijn MF, Voerman JS, Campbell PA, van Ewijk W. Markers of mouse macrophage development detected by monoclonal antibodies. J Immunol Methods. 1994; 174(1-2):5-19. (Clone-specific). View Reference
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Ravishankar B, Liu H, Shinde R, et al. The amino acid sensor GCN2 inhibits inflammatory responses to apoptotic cells promoting tolerance and suppressing systemic autoimmunity.. Proc Natl Acad Sci U S A. 2015; 112(34):10774-9. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). View Reference
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Van Vliet E, Melis M, Van Ewijk W. Monoclonal antibodies to stromal cell types of the mouse thymus.. Eur J Immunol. 1984; 14(6):524-9. (Immunogen: Immunohistochemistry). View Reference
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van Vliet E, Melis M, van Ewijk W. Marginal zone macrophages in the mouse spleen identified by a monoclonal antibody. Anatomical correlation with a B cell subpopulation.. J Histochem Cytochem. 1985; 33(1):40-4. (Immunogen: Immunohistochemistry). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.