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      PE Mouse Anti-Human TCR Vβ23

      BD Pharmingen™ PE Mouse Anti-Human TCR Vβ23

      Clone AHUT7 (also known as AF23; AF-23; αHUT7; HUT-78; HUT78#7)

      (RUO)
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      PE Mouse Anti-Human TCR Vβ23
      Two-color flow cytometric analysis of TCR Vβ23 expression on human peripheral blood T lymphocytes. Human peripheral blood cells were stained with APC Mouse Anti-Human CD3 antibody (Cat. No. 561811) and with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-Human TCR Vβ23 antibody (Cat. No. 568573/568574; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The bivariate pseudocolor density plot showing the correlated expression of TCR Vβ23 (or Ig Isotype control staining) versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
      PE Mouse Anti-Human TCR Vβ23
      Two-color flow cytometric analysis of TCR Vβ23 expression on human peripheral blood T lymphocytes. Human peripheral blood cells were stained with APC Mouse Anti-Human CD3 antibody (Cat. No. 561811) and with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-Human TCR Vβ23 antibody (Cat. No. 568573/568574; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The bivariate pseudocolor density plot showing the correlated expression of TCR Vβ23 (or Ig Isotype control staining) versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
      Product Details
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      BD Pharmingen™
      TCR V beta 23; TCRBV23S1; TRBV13; TRBV23-1
      Human (QC Testing)
      Mouse BALB/c IgG1, κ
      HUT78 T Cell Leukemia Line
      Flow cytometry (Routinely Tested)
      5 µl
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      3. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      4. An isotype control should be used at the same concentration as the antibody of interest.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
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      568574 Rev. 2
      Antibody Details
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      AHUT7

      The AHUT7 monoclonal antibody specifically recognizes the human variable beta 23 region of the β subunit of the human αβ T cell receptor for antigen (TCR Vβ23). The  TCR Vβ23 region is encoded by the TRBV13 gene segment. TCR Vβ23 is expressed on subsets of TCR αβ positive thymocytes and on ~0.3 to 5% of peripheral blood T cells including CD4+ and CD8+ T cells. The AHUT7 antibody is useful for multiparameter analyses designed to study the nature of TCR Vβ23-positive T cells including normal T cells as well as T cell clones, hybridomas, or tumor cell lines including H9 and HUT-78. It is also useful for analyzing TCR Vβ repertoires expressed by T cell populations collected from blood, tissues or other sources in health and disease models including inflammation, autoimmunity, responses to superantigens, tumors, and infectious diseases. The AHUT7 antibody can reportedly stimulate the proliferation of TCR Vβ23-positive T cells.

      568574 Rev. 2
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      568574 Rev.2
      Citations & References
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      View product citations for antibody "568574" on CiteAb

      Development References (8)

      1. Beres SB, Sylva GL, Barbian KD, et al. Genome sequence of a serotype M3 strain of group A Streptococcus: phage-encoded toxins, the high-virulence phenotype, and clone emergence.. Proc Natl Acad Sci U S A. 2002; 99(15):10078-83. (Biology). View Reference
      2. Ghia P, Prato G, Stella S, Scielzo C, Geuna M, Caligaris-Cappio F. Age-dependent accumulation of monoclonal CD4+CD8+ double positive T lymphocytes in the peripheral blood of the elderly.. Br J Haematol. 2007; 139(5):780-90. (Clone-specific: Flow cytometry). View Reference
      3. Giudice V, D'Addona M, Montuori N, Selleri C. The Value of Flow Cytometry Clonality in Large Granular Lymphocyte Leukemia.. Cancers (Basel). 2021; 13(18):4513. (Clone-specific: Flow cytometry). View Reference
      4. Guilherme L, Faé KC, Oshiro SE, Tanaka AC, Pomerantzeff PM, Kalil J. T cell response in rheumatic fever: crossreactivity between streptococcal M protein peptides and heart tissue proteins.. Curr Protein Pept Sci. 2007; 8(1):39-44. (Biology). View Reference
      5. Lavoie PM, Dumont AR, McGrath H, Kernaleguen AE, Sékaly RP. Delayed expansion of a restricted T cell repertoire by low-density TCR ligands.. Int Immunol. 2005; 17(7):931-41. (Clone-specific: Flow cytometry). View Reference
      6. Pantaleo G, Soudeyns H, Demarest JF, et al. Evidence for rapid disappearance of initially expanded HIV-specific CD8+ T cell clones during primary HIV infection.. Proc Natl Acad Sci U S A. 1997; 94(18):9848-53. (Clone-specific: Fluorescence activated cell sorting). View Reference
      7. Rebai N, Pantaleo G, Demarest JF, et al. Analysis of the T-cell receptor beta-chain variable-region (V beta) repertoire in monozygotic twins discordant for human immunodeficiency virus: evidence for perturbations of specific V beta segments in CD4+ T cells of the virus-positive twins.. Proc Natl Acad Sci U S A. 1994; 91(4):1529-33. (Clone-specific: Flow cytometry). View Reference
      8. Romagné F, Kanagawa O, David-Ameline J, Peyrat MA, Bonneville M, Necker A. TCRBV23 specificity of two monoclonal antibodies revealed by a panel of human V beta chains expressed in mouse cells.. J Immunol Methods. 1995; 186(2):313-22. (Immunogen: Flow cytometry). View Reference
      View All (8) View Less
      568574 Rev. 2

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.