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      PE Rat Anti-Mouse DCIR4 (Clec4a1)
      PE Rat Anti-Mouse DCIR4 (Clec4a1)
      Two-color flow cytometric analysis of DCIR4 (Clec4a1) expression on mouse splenic leucocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with BD Horizon™ BV421 Rat Anti-CD11b antibody (Cat. No. 562605) and either PE Rat IgG1, κ Isotype Control (Cat. No. 553925; Left Plot) or PE Rat Anti-Mouse DCIR4 (Clec4a1) antibody (Cat. No. 567034; Right Plot) at 0.5 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of DCIR4 (Clec4a1) [or Ig Isotype control staining] versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable (7-AAD-negative) leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      PE Rat Anti-Mouse DCIR4 (Clec4a1)
      Two-color flow cytometric analysis of DCIR4 (Clec4a1) expression on mouse splenic leucocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with BD Horizon™ BV421 Rat Anti-CD11b antibody (Cat. No. 562605) and either PE Rat IgG1, κ Isotype Control (Cat. No. 553925; Left Plot) or PE Rat Anti-Mouse DCIR4 (Clec4a1) antibody (Cat. No. 567034; Right Plot) at 0.5 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of DCIR4 (Clec4a1) [or Ig Isotype control staining] versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable (7-AAD-negative) leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      Product Details
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      BD Pharmingen™
      Clec4a1; DCIR4; Dcir4; dendritic cell inhibitory receptor 4
      Mouse (QC Testing)
      Rat LEW, also known as Lewis IgG1, κ
      Mouse DCIR4
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      AB_2870015
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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      Recommended Assay Procedures

      BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      567034 Rev. 1
      Antibody Details
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      MH7E7

      The MH7E7 monoclonal antibody specifically recognizes the extracellular domain of mouse Dendritic cell inhibitory receptor 4 (DCIR4). DCIR4 is a lectin receptor that is encoded by Clec4a1 (C-type lectin domain family 4, member a1) which belongs to the dendritic cell immunoreceptor (DCIR) family. DCIR4 (Clec4a1) is variably expressed on CD11b+ monocytes found in peripheral blood, secondary lymphoid tissues and the bone marrow. DCIR4 (Clec4a1) is reportedly expressed at low levels on Ly-6C-positive inflammatory monocytes and at high levels on Ly-6C-negative patrolling monocytes. It is not significantly expressed on eosinophils and neutrophils.

              

      567034 Rev. 1
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      567034 Rev.1
      Citations & References
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      View product citations for antibody "567034" on CiteAb

      Development References (3)

      1. Flornes LM, Bryceson YT, Spurkland A, Lorentzen JC, Dissen E, Fossum S. Identification of lectin-like receptors expressed by antigen presenting cells and neutrophils and their mapping to a novel gene complex.. Immunogenetics. 2004; 56(7):506-17. (Biology). View Reference
      2. Hsu Y, Okada R, Nishimura T, Kawasaki N, Yamamoto K, Matsumoto N. DCIR3 and DCIR4 are co-expressed on inflammatory and patrolling monocytes. Biochem Biophys Res Commun.. 2017; 494(3-4):440-445. (Clone-specific: Flow cytometry). View Reference
      3. Kameda Y, Hanayama M, Kishimoto A, Kume M, Yamamoto K, Matsumoto N. Dendritic cell inhibitory receptor 4 (DCIR4) is preferentially expressed on inflammatory and patrolling monocytes.. Biochem Biophys Res Commun. 2016; 480(2):215-221. (Immunogen: Flow cytometry). View Reference
      567034 Rev. 1

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.