PE Mouse Anti-Human CD245

BD Pharmingen™ PE Mouse Anti-Human CD245

Name: PE Mouse Anti-Human CD245
Brand: BD Pharmingen™
SKU: 566886

Clone DY12

(RUO)

Multiparameter flow cytometric analysis of CD245 expression on human peripheral blood leucocyte populations. Human whole blood was stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse anti-Human CD245 antibody (Cat. No. 566886; Right Plot). Erythrocytes were then lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The two-parameter pseudocolor density plot showing the correlated expression of CD245 (or Ig Isotype control staining) versus side light-scatter signals was derived from gated events with the light scattering characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa™ Flow Cytometer System and FlowJo™ software.

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Product Details

Brand: BD Pharmingen™

Alternative Name: MYO18A; myosin 18A; unconventional myosin-XVIIIa; MAJN; MYSPDZ

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human NK Cell Line

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl

Workshop Number: VI 6T-021; VII 70008; X 10-43

RRID: AB_2869937

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Lysing Buffer RUO

Size 100 mL Cat No. 555899

Lysing Solution 10X Concentrate IVD

Size 100 mL Cat No. 349202

PE Mouse IgG1, κ Isotype Control RUO

Size 0.1 mg Cat No. 554680

566886 Rev. 1

Antibody Details

DY12

The DY12 monoclonal antibody specifically recognizes CD245 which is also known as p220/240. CD245 is variably expressed by T cells, B cells. NK cells, granulocytes, and platelets and more highly expressed on monocytes. CD245 expression can be upregulated on activated T cells, B cells, and NK cells. CD245 has been identified as Myosin 18A that is encoded by MYO18A (Myosin XVIIIA). Myosin 18A is also known as Myosin containing a PDZ domain (MysPDZ), Molecule associated with JAK3 N-terminus (MAJN), and Surfactant protein receptor SP-R2101 (SP-R2101). Myosin 18A is involved in intracellular transport processes and can also reportedly serve as a cell surface receptor for Surfactant Protein A (SP-A). The DY12 antibody reportedly immunoprecipitated long α (230 kDa) and short β (190 kDa) isoforms of Myosin 18A expressed by normal human lung extracts and by the YT2C2 NK cell line-further evidence suggested that the Myosin 18Aα isoform is expressed at the cell surface. CD245 stimulation mediated by bound and crosslinked DY12 antibody or the SP-A ligand can reportedly augment NK cell degranulation or IL-2-activated NK cell-mediated cytotoxicity against certain tumor target cells. Moreover, stimulation through CD245 can upregulate CD137/4-1BB expression on NK cells and promote NK cell-mediated cytotoxicity against CD137 Ligand-expressing target cells.

566886 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

566886 Rev.1

Citations & References

View product citations for antibody "566886" on CiteAb

Development References (5)

Boumsell L, Nikolova M, Marie-Cardine A, Bensussan A. CD245 Workshop report. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:691-692.
De Masson A, Giustiniani J, Marie-Cardine A, et al. Identification of CD245 as myosin 18A, a receptor for surfactant A: A novel pathway for activating human NK lymphocytes.. Oncoimmunology. 2016; 5(5):e1127493. (Immunogen: Flow cytometry, Functional assay, Immunohistochemistry). View Reference
Gurka S, Dirks S, Photiadis J, Kroczek RA. Expression analysis of surface molecules on human thymic dendritic cells with the 10th HLDA Workshop antibody panel.. Clin Transl Immunology. 2015; 4(10):e47. (Clone-specific: Flow cytometry). View Reference
Okumura K, Kato K, Kobata T. T-cell antigens: Section report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:23-32.
Zola H, Swart B, Boumsell L, Mason DY. Human Leucocyte Differentiation Antigen nomenclature: update on CD nomenclature. Report of IUIS/WHO Subcommittee.. J Immunol Methods. 2003; 275(1-2):1-8. (Clone-specific). View Reference

View All (5)

566886 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.