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      Alexa Fluor® 647 Mouse Anti-Human Transferrin R (CD71)

      BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Human Transferrin R (CD71)

      Clone OKT9 (also known as OKT-9; OKT 9)

      (RUO)
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      Alexa Fluor® 647 Mouse Anti-Human Transferrin R (CD71)
      Flow cytometric analysis of Transferrin R (CD71) expression on activated human peripheral blood mononuclear cells (PBMC). Human PBMC were stimulated for 3 days with Phytohemagglutinin (PHA). The cells were harvested, preincubated with Human BD Fc Block™ (Cat. No. 564219/564220), and then stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 565571; dashed line histogram) or Alexa Fluor® 647 Mouse Anti-Human Transferrin R (CD71) antibody (Cat. No. 566724/566725; solid line histogram). BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The fluorescence histogram showing CD71 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable activated human peripheral mononuclear cells. Flow cytometry and data analysis were performed using a BD FACSCelesta™ Flow Cytometer System and FlowJo™ software.
      Alexa Fluor® 647 Mouse Anti-Human Transferrin R (CD71)
      Flow cytometric analysis of Transferrin R (CD71) expression on activated human peripheral blood mononuclear cells (PBMC). Human PBMC were stimulated for 3 days with Phytohemagglutinin (PHA). The cells were harvested, preincubated with Human BD Fc Block™ (Cat. No. 564219/564220), and then stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 565571; dashed line histogram) or Alexa Fluor® 647 Mouse Anti-Human Transferrin R (CD71) antibody (Cat. No. 566724/566725; solid line histogram). BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The fluorescence histogram showing CD71 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable activated human peripheral mononuclear cells. Flow cytometry and data analysis were performed using a BD FACSCelesta™ Flow Cytometer System and FlowJo™ software.
      Product Details
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      BD Pharmingen™
      TfR; sTfR; TFRC; Trfr; TfR1; TR; T9; p90
      Human (QC Testing)
      Mouse BALB/c x A/J, also known as CAF1 IgG1, κ
      Human T-cell acute lymphoblastic leukemia cells
      Flow cytometry (Routinely Tested)
      5 µl
      AB_2869830
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
      6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      7. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      566725 Rev. 1
      Antibody Details
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      OKT9

      The OKT9 monoclonal antibody specifically binds to CD71 which is also known as the transferrin receptor (TFR or Transferrin R). CD71 (Transferrin R) is a type II transmembrane glycoprotein that is encoded by TFRC (transferrin receptor) which belongs to the Transferrin receptor family.  It is expressed on cells as a disulfide-linked homodimer comprised of ~95 kDa monomers. CD71 is expressed on early thymocytes as well as activated lymphocytes, monocytes, macrophages, brain endothelium, and most proliferating cells. The transferrin receptor is also present on early erythroid cells but is lost as reticulocytes differentiate into mature erythrocytes. CD71 is lowly expressed on normal resting lymphocytes and is upregulated during lymphocyte responses to antigens or mitogens. The transferrin receptor is essential for iron transport into proliferating cells. Through an endocytic pathway, the transferrin receptor mediates cellular iron uptake by binding and internalizing iron that is bound to transferrin. After releasing iron within the low pH endosomal environment, transferrin and its receptor can be recycled to the cell surface.

              

      566725 Rev. 1
      Format Details
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      Alexa Fluor™ 647
      Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 647
      Red 627-640 nm
      653 nm
      669 nm
      566725 Rev.1
      Citations & References
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      View product citations for antibody "566725" on CiteAb

      Development References (4)

      1. Goding JW, Burns GF. Monoclonal antibody OKT-9 recognizes the receptor for transferrin on human acute lymphocytic leukemia cells. J Immunol. 1981; 127(3):1256-1258. (Clone-specific: Immunoprecipitation). View Reference
      2. Reinherz EL, Kung PC, Goldstein G, Levey RH, Schlossman SF. Discrete stages of human intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage. Proc Natl Acad Sci U S A. 1980; 77(3):1588-1592. (Immunogen: Flow cytometry). View Reference
      3. Schwarting R, Stein H. Cluster report: CD71. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:455-460.
      4. Zola H, Swart B, Nicholson I, Voss E. CD71. In: Zola H. Leukocyte and Stromal Cell Molecules : the CD Markers. Hoboken, N.J.: Wiley-Liss; 2007:163.
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      566725 Rev. 1

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.