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BV421 Mouse Anti-Human CD86
BV421 Mouse Anti-Human CD86
Flow cytometric analysis of CD86 expression on Human Daudi cells. Cells from the human Daudi (Burkitt's lymphoma, ATCC CRL-213) cell line were stained with either BD Horizon™ BV421Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histogram) or BD Horizon BV421 Mouse Anti-Human CD86 antibody (Cat. No. 566454; solid line histogram) at 0.25 μg/test. The fluorescence histogram showing CD86 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
BV421 Mouse Anti-Human CD86
Multiparameter flow cytometric analysis of CD86 expression on human peripheral blood leucocytes. Human whole blood was stained with either BD Horizon™ BV421Mouse IgG1, κ Isotype Control (Cat. No. 562438; Left Plot) or BD Horizon BV421 Mouse Anti-Human CD86 antibody (Cat. No. 566454; Right Plot) at 0.25 μg/test.  Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899).Two-parameter flow cytometric contour plots showing the correlated expression of CD86 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific
Flow cytometric analysis of CD86 expression on Human Daudi cells. Cells from the human Daudi (Burkitt's lymphoma, ATCC CRL-213) cell line were stained with either BD Horizon™ BV421Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histogram) or BD Horizon BV421 Mouse Anti-Human CD86 antibody (Cat. No. 566454; solid line histogram) at 0.25 μg/test. The fluorescence histogram showing CD86 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Multiparameter flow cytometric analysis of CD86 expression on human peripheral blood leucocytes. Human whole blood was stained with either BD Horizon™ BV421Mouse IgG1, κ Isotype Control (Cat. No. 562438; Left Plot) or BD Horizon BV421 Mouse Anti-Human CD86 antibody (Cat. No. 566454; Right Plot) at 0.25 μg/test.  Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899).Two-parameter flow cytometric contour plots showing the correlated expression of CD86 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific
Product Details
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BD Horizon™
B7.2; B7-2; B-lymphocyte activation antigen B7-2; B70; CD28LG2; LAB72
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human ARH 77 Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
V BP072, A109; VI BP95, CD86.9
AB_2739733
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
  7. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566454 Rev. 1
Antibody Details
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BU63

The BU63 monoclonal antibody specifically recognizes CD86 which is also known as B70, B7-2, B7.2, LAB72 or CD28LG2. CD86 is a ~70-80 kDa type I transmembrane glycoprotein that belongs to the Ig gene superfamily. CD86 is expressed on monocytes, dendritic cells, Langerhans cells, activated B cells, and memory B cells. Like CD80 (B7-1), CD86 serves as a ligand for CD28 or CD152 (CTLA-4) expressed by T cells and can induce costimulatory or coinhibitory signals, respectively. The BU63 antibody can reportedly block the binding of other human CD86-specific antibodies including the 2331 (FUN-1) (complete block) or IT2.2 (partial block) antibodies. These results suggest that the BU63 antibody may bind to a similar or identical CD86 epitope as the 2331 (FUN-1) antibody but to a different, although spatially-related epitope compared with the IT2.2 antibody.

The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

566454 Rev. 1
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
566454 Rev.1
Citations & References
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View product citations for antibody "566454" on CiteAb

Development References (5)

  1. Azuma M, Ito D, Yagita H, et al. B70 antigen is a second ligand for CTLA-4 and CD28.. Nature. 1993; 366(6450):76-9. (Biology). View Reference
  2. Engel P, Gribben JG, Freeman GJ, et al. The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen. Blood. 1994; 84(5):1402-1407. (Clone-specific: Blocking, Flow cytometry). View Reference
  3. Engel P, Wagner N, Tedder TF. CD86 Workshop Report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:703-705.
  4. Hardie DL, Casamayor M, Johnson GD, et al. CD86 Workshop Panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:201-204.
  5. Nozawa Y, Abe M, Wakasa H. Three mAb, FUN-1, FB1, and FB21, that recognize B-cell antigens in frozen or paraffin-embedded tissue sections. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:705-706.
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566454 Rev. 1

 

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.