.png){kind=spacer}
Preparation And Storage
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
The NaM185-2C3 monoclonal antibody specifically recognizes CD234 which is also known as the Duffy antigen/receptor for chemokines (DARC), Fy glycoprotein (FY), or Glycoprotein D (GPD). CD234 is a seven-transmembrane domain acidic glycoprotein that is encoded by ACKR1 [atypical chemokine receptor 1 (Duffy blood group)] and belongs to the chemokine receptor superfamily. CD234 is responsible for the Duffy blood group system and is expressed on erythrocytes, endothelial cells, and some epithelial cells and Purkinje cells. CD234 binds to multiple chemokines including CXCL1 (also known as, MGSA or GROa), CXCL8 (IL-8), CCL2 (MCP-1), and CCL5 (RANTES). Because CD234 is not a signaling receptor, it may act as a decoy receptor or scavenger and serve to control chemokine levels. CD234 may also serve to regulate the availability of chemokines such as when it is expressed by endothelial cells at inflammatory sites or by postcapillary endothelial cells. CD234 can mediate chemokine transcytosis from the basolateral to the luminal side of the endothelium, where the bound chemokines are "presented" to regulate leucocyte trafficking. CD234 functions as a receptor for malarial parasites including Plasmodium vivax and Plasmodium knowlesi. Some individuals with mutations that prevent CD234 expression in erythrocytes are resistant to infection by these pathogens. The NaM185-2C3 antibody reportedly detects a protease-sensitive determinant and inhibits IL-8 binding to the Duffy antigen.
Development References (9)
-
Blanchard D, Petit-Le Roux Y, Tournamille C, Folléa G. Characterization of monoclonal antibodies directed against the Fy/DARC erythrocyte glycoprotein. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:574-576. View Reference
-
Geoff Daniels. Duffy Blood Group System. In: Daniels G. Human blood groups : Geoff Daniels ; foreword by Ruth Sanger., 3rd ed.. Chichester, West Sussex: John Wiley & Sons; 2013:306.
-
Horuk R, Chitnis CE, Darbonne WC, et al. A receptor for the malarial parasite Plasmodium vivax: the erythrocyte chemokine receptor.. Science. 1993; 261(5125):1182-4. (Biology). View Reference
-
Nichols ME, Rubinstein P, Barnwell J, Rodriguez de Cordoba S, Rosenfield RE. A new human Duffy blood group specificity defined by a murine monoclonal antibody. Immunogenetics and association with susceptibility to Plasmodium vivax.. J Exp Med. 1987; 166(3):776-85. (Biology). View Reference
-
Riwom S, Janvier D, Navenot JM, Benbunan M, Muller JY, Blanchard D. Production of a new murine monoclonal antibody with Fy6 specificity and characterization of the immunopurified N-glycosylated Duffy-active molecule.. Vox Sang. 1994; 66(1):61-7. (Biology). View Reference
-
Van der Schoot CE, Baardman R, Lighthart P, de Jong I, Eg Von Dem Borne A, de Haas M. CD234 - DARC. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:573-574.
-
Walker RH, ed. Technical Manual of the American Association of Blood Banks. Arlington VA: 1990.
-
Wasniowska K, Petit-LeRoux Y, Tournamille C, et al. Structural characterization of the epitope recognized by the new anti-Fy6 monoclonal antibody NaM 185-2C3.. Transfus Med. 2002; 12(3):205-11. (Immunogen: Blocking, ELISA, Flow cytometry, Functional assay, Hemagglutination assay, Inhibition, Western blot). View Reference
-
Zola H, Swart B, Nicholson I, Voss E. CD234. In: Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007:387.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.