Two-color flow cytometric analysis of IL31RA expression on human peripheral blood mononuclear cells. Left Panel - Ex vivo human peripheral blood mononuclear cells (PBMCs) were stained with BD Horizon™ BV421 Mouse Anti-Human CD14 antibody (Cat. No. 565283) and either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Cat. No. 557690; Top Plot) or Alexa Fluor® 647 Rat Anti-Human IL-31RA antibody (Cat. No. 566351; Bottom Plot) at 0.25 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. Right Panel - PBMCs from the same donor were cultured in complete tissue culture medium without (Left Plots) or with (Right Plots) Recombinant Human IFN-γ protein (Cat. No. 554617; 50 ng/ml, 20 h, 37°C). The cells were harvested, and similarly stained with BV421 Mouse Anti-Human CD14 antibody with either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Top Plots) or Alexa Fluor® 647 Rat Anti-Human IL-31RA antibody (Bottom Plots) at 0.25 µg/test, followed by viability staining with BD Via-Probe™ Cell Viability 7-AAD Solution. Two-color flow cytometric contour plots showing the correlated expression of CD14 versus IL-31RA (or Ig Isotype control staining) were derived from 7-AAD negative-gated events with the forward and side light-scatter characteristics of viable PBMCs. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Two-color flow cytometric analysis of IL31RA expression on human peripheral blood mononuclear cells. Left Panel - Ex vivo human peripheral blood mononuclear cells (PBMCs) were stained with BD Horizon™ BV421 Mouse Anti-Human CD14 antibody (Cat. No. 565283) and either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Cat. No. 557690; Top Plot) or Alexa Fluor® 647 Rat Anti-Human IL-31RA antibody (Cat. No. 566351; Bottom Plot) at 0.25 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. Right Panel - PBMCs from the same donor were cultured in complete tissue culture medium without (Left Plots) or with (Right Plots) Recombinant Human IFN-γ protein (Cat. No. 554617; 50 ng/ml, 20 h, 37°C). The cells were harvested, and similarly stained with BV421 Mouse Anti-Human CD14 antibody with either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Top Plots) or Alexa Fluor® 647 Rat Anti-Human IL-31RA antibody (Bottom Plots) at 0.25 µg/test, followed by viability staining with BD Via-Probe™ Cell Viability 7-AAD Solution. Two-color flow cytometric contour plots showing the correlated expression of CD14 versus IL-31RA (or Ig Isotype control staining) were derived from 7-AAD negative-gated events with the forward and side light-scatter characteristics of viable PBMCs. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Product Details
BD Pharmingen™
IL31RA; IL-31R-alpha; GLMR; GLM-R; hGLM-R; CRL3
Human (QC Testing)
Rat LEW, also known as Lewis IgG2a, κ
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2739705
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Alexa Fluor® 647 Rat IgG2a, κ Isotype Control RUO
Size
0.1 mg
Cat No.
557690
Cell Viability Solution RUO
Size
500 Tests
Cat No.
555815
Cell Viability Solution RUO
Size
100 Tests
Cat No.
555816
Recombinant Human IFN-γ RUO
Size
50 µg
Cat No.
554617
566351 Rev. 1
Antibody Details
V1-1110
The V1-1110 monoclonal antibody specifically recognizes the Interleukin-31 Receptor subunit alpha (IL-31R alpha, IL-31RA, or IL-31Rα) which is also known as gp130-like Monocyte Receptor (GLMR or hGLM-R) or Cytokine receptor-like 3 (CRL3). IL-31RA is a type I transmembrane glycoprotein that is encoded by IL31RA and is classified as a type I cytokine receptor. IL-31RA associates with the Oncostatin M Receptor (OSMR) to form a heterodimeric signaling receptor for IL-31. Through this heterodimeric receptor, IL-31 can elicit intracellular JAK/STAT (including activated Stat3, Stat5, or Stat1), RAS/ERK and PI3K/AKT signal transduction pathways. IL-31RA is variably expressed by monocytes, dendritic cells, keratinocytes, and epithelial cells. IL-31RA and OSMR are inducibly expressed by cells within the myelomonocytic lineage such as monocytes treated with IFN-γ and bacterial lipopolysaccharide (LPS). IL-31RA signaling plays important roles in both innate and adaptive immunity and inflammation in tissues that are in close contact with the environment including the skin, lung, and intestines.
566351 Rev. 1
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
566351 Rev.1
Citations & References
View product citations for antibody "566351" on CiteAb
Development References (5)
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Cornelissen C, Lüscher-Firzlaff J, Baron JM, Lüscher B. Signaling by IL-31 and functional consequences.. Eur J Cell Biol. 91(6-7):552-66. (Biology). View Reference
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Dillon SR, Sprecher C, Hammond A, et al. Interleukin 31, a cytokine produced by activated T cells, induces dermatitis in mice.. Nat Immunol. 2004; 5(7):752-60. (Biology). View Reference
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Edukulla R, Singh B, Jegga AG, Sontake V, Dillon SR, Madala SK. Th2 Cytokines Augment IL-31/IL-31RA Interactions via STAT6-dependent IL-31RA Expression.. J Biol Chem. 2015; 290(21):13510-20. (Biology). View Reference
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Ghilardi N, Li J, Hongo JA, Yi S, Gurney A, de Sauvage FJ. A novel type I cytokine receptor is expressed on monocytes, signals proliferation, and activates STAT-3 and STAT-5.. J Biol Chem. 2002; 277(19):16831-6. (Biology). View Reference
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Zhang Q, Putheti P, Zhou Q, Liu Q, Gao W. Structures and biological functions of IL-31 and IL-31 receptors.. Cytokine Growth Factor Rev. 19(5-6):347-56. (Biology). View Reference
566351 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.