Preparation And Storage
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
- BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
The RMT1-4 monoclonal antibody specifically recognizes CD365 which is encoded by Havcr1 (Hepatitis A virus cellular receptor 1) and commonly known as T cell immunoglobulin and mucin domain-containing protein 1 (TIM-1). TIM-1 is a type I transmembrane glycoprotein that contains an extracellular immunoglobulin V-like domain and mucin-like domain and a conserved intracellular tyrosine phosphorylation motif that is involved in transmembrane signaling. TIM-1 is a costimulatory molecule that plays an important role in regulating immune responses. It is expressed on mast cells, and activated T cells including T helper type 2 (Th2) cells. TIM-1 is constitutively expressed by some B cells with increased TIM-1 expression detected upon B cell activation. TIM-1 was reportedly expressed by the majority of IL-10-expressing regulatory B cells within several major splenic B cell subsets. These cells may play critical roles in Th2-like CD4 T cell differentiation and in the regulation of inflammation and autoimmunity. TIM-4, a phosphatidylserine receptor encoded by Timd4, is a putative ligand for TIM-1.
Development References (5)
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Ding Q, Yeung M, Camirand G, et al. Regulatory B cells are identified by expression of TIM-1 and can be induced through TIM-1 ligation to promote tolerance in mice.. J Clin Invest. 2011; 121(9):3645-56. (Clone-specific: Flow cytometry). View Reference
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Kuchroo VK, Umetsu DT, DeKruyff RH, Freeman GJ. The TIM gene family: emerging roles in immunity and disease.. Nat Rev Immunol. 2003; 3(6):454-62. (Biology). View Reference
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Ma J, Usui Y, Takeda K, et al. TIM-1 signaling in B cells regulates antibody production.. Biochem Biophys Res Commun. 2011; 406(2):223-8. (Biology: Flow cytometry). View Reference
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Nakae S, Iikura M, Suto H, et al. TIM-1 and TIM-3 enhancement of Th2 cytokine production by mast cells.. Blood. 2007; 110(7):2565-8. (Immunogen: Flow cytometry). View Reference
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Umetsu SE, Lee WL, McIntire JJ, et al. TIM-1 induces T cell activation and inhibits the development of peripheral tolerance.. Nat Immunol. 2005; 6(5):447-54. (Biology). View Reference
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