Skip to main content Skip to navigation
PE Mouse Anti-Human CD31
PE Mouse Anti-Human CD31
Flow cytometric analysis of human CD31 expression on human peripheral blood leucocytes. Human whole blood was stained with either PE Mouse IgG1, κ Isotype Control (Cat. No.554680; dashed line histograms) or PE Mouse Anti-Human CD31 antibody (Cat. No. 566125/566177; solid line histograms). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899).The fluorescence histograms showing CD31 expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable monocytes (Left Panel) or granulocytes (Right Panel). Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Flow cytometric analysis of human CD31 expression on human peripheral blood leucocytes. Human whole blood was stained with either PE Mouse IgG1, κ Isotype Control (Cat. No.554680; dashed line histograms) or PE Mouse Anti-Human CD31 antibody (Cat. No. 566125/566177; solid line histograms). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899).The fluorescence histograms showing CD31 expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable monocytes (Left Panel) or granulocytes (Right Panel). Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
Product Details
Down Arrow Up Arrow


BD Pharmingen™
PECAM-1; PECAM1; EndoCAM; GPIIA'
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human CD31
Flow cytometry (Routinely Tested)
5 µl
V P112
AB_2739574
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  6. An isotype control should be used at the same concentration as the antibody of interest.
566125 Rev. 1
Antibody Details
Down Arrow Up Arrow
MBC 78.2

The MBC 78.2 monoclonal antibody recognizes CD31 which is also known as, Platelet endothelial cell adhesion molecule (PECAM-1), platelet GPIIa, or EndoCAM. CD31 is a ~130 kDa type I transmembrane glycoprotein that belongs to the Ig gene superfamily. CD31 is comprised of an extracellular region with six IgC-like domains, a transmembrane region, and a cytoplasmic domain that contains two immunoreceptor tyrosine-based inhibitory motifs (ITIMs).  The MBC 78.2 antibody specifically binds to an epitope located on membrane-proximal, extracellular Ig-like domain 6 of CD31. This epitope remains expressed by activated T cells after enzymatic cleavage and shedding of a soluble extracellular CD31 fragment comprised of Ig-like domains 1 to 5 from cells. In contrast to the MBC 78.2 antibody, the WM59 monoclonal antibody reportedly binds to the extracellular Ig-like domain 2 of CD31. WM59 can thus bind to cells that express intact CD31 but not to cells that express a truncated form CD31 that lacks at least the membrane distal Ig-like domains 1 and 2 of CD31. CD31 has wide tissue distribution and is expressed on platelets, monocytes, granulocytes, some T cell subsets, and at high levels on endothelial cells. This cell adhesion molecule has been implicated in a number of cellular phenomena, including vascular wound healing. angiogenesis, transendothelial migration of leucocytes, and the regulation of T cell responses.

        

566125 Rev. 1
Format Details
Down Arrow Up Arrow
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566125 Rev.1
Citations & References
Down Arrow Up Arrow
View product citations for antibody "566125" on CiteAb

Development References (7)

  1. DeLisser HM, Chilkotowsky J, Yan HC, Daise ML, Buck CA, Albelda SM. Deletions in the cytoplasmic domain of platelet-endothelial cell adhesion molecule-1 (PECAM-1, CD31) result in changes in ligand binding properties.. J Cell Biol. 1994; 124(1-2):195-203. (Immunogen: Flow cytometry, Functional assay, Inhibition). View Reference
  2. Fawcett J, Buckley C, Holness CL, et al. Mapping the homotypic binding sites in CD31 and the role of CD31 adhesion in the formation of interendothelial cell contacts. J Cell Biol. 1995; 128(6):1229-1241. (Biology). View Reference
  3. Fornasa G, Groyer E, Clement M, et al. TCR stimulation drives cleavage and shedding of the ITIM receptor CD31. J Immunol. 2010; 184(10):5485-5492. (Clone-specific: Cytometric Bead Array, Flow cytometry, Immunofluorescence). View Reference
  4. Newman PJ, Paddock C. CD31 cluster workshop report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1259-1260.
  5. Tanaka Y, Albelda SM, Horgan KJ, et al. CD31 expressed on distinctive T cell subsets is a preferential amplifier of beta 1 integrin-mediated adhesion.. J Exp Med. 1992; 176(1):245-53. (Clone-specific: Activation, Functional assay). View Reference
  6. Yan H-C, Newman PJ, Albelda SM. Epitope mapping of CD31 (PECAM-1) mAb. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1261-1262.
  7. Yan HC, Pilewski JM, Zhang Q, DeLisser HM, Romer L, Albelda SM. Localization of multiple functional domains on human PECAM-1 (CD31) by monoclonal antibody epitope mapping.. Cell Adhes Commun. 1995; 3(1):45-66. (Clone-specific: Immunoprecipitation). View Reference
View All (7) View Less
566125 Rev. 1

 

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.