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      Alexa Fluor® 647 Mouse Anti-Human Neuropilin-1 (CD304)
      Alexa Fluor® 647 Mouse Anti-Human Neuropilin-1 (CD304)
      Two-color flow cytometric analysis of Neuropilin-1 (CD304) expression on human peripheral blood lymphoid cells. Human peripheral blood mononuclear cells were stained with BD Horizon™ BV421 Mouse Anti-Human CD123 antibody (Cat. No. 562517/565928) and either Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (Cat. No. 557691; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human Neuropilin-1 (CD304) antibody (Cat. No. 566047/566048; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD123 versus Neuropilin-1 (CD304) [or Ig Isotype control staining] were derived from gated events with the forward and side light-scatter characteristics of viable lymphoid cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
      Alexa Fluor® 647 Mouse Anti-Human Neuropilin-1 (CD304)
      Two-color flow cytometric analysis of Neuropilin-1 (CD304) expression on human peripheral blood lymphoid cells. Human peripheral blood mononuclear cells were stained with BD Horizon™ BV421 Mouse Anti-Human CD123 antibody (Cat. No. 562517/565928) and either Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (Cat. No. 557691; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human Neuropilin-1 (CD304) antibody (Cat. No. 566047/566048; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD123 versus Neuropilin-1 (CD304) [or Ig Isotype control staining] were derived from gated events with the forward and side light-scatter characteristics of viable lymphoid cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
      Product Details
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      BD Pharmingen™
      Neuropilin; NRP; NP1; Blood dendritic cell antigen 4; BDCA-4; VEGF165R
      Human (QC Testing)
      Mouse IgG2b, κ
      Recombinant Human Neuropilin-1
      Flow cytometry (Routinely Tested)
      5 µl
      AB_2744357
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
      6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      7. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      566048 Rev. 1
      Antibody Details
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      U21-1283

      The U21-1283 monoclonal antibody specifically recognizes Neuropilin-1 (NRP1), also known as CD304, Blood dendritic cell antigen 4 (BDCA4), and Vascular endothelial cell growth factor 165 receptor (VEGF165R). CD304 is a type I transmembrane glycoprotein involved in the development of the nervous and cardiovascular systems. It mediates the interaction, growth, survival, and migration of a variety of normal and tumor cells. CD304 is expressed on neurons, thymocytes, regulatory T cells, a subset of T follicular helper cells, dendritic cells, endothelial cells, and certain tumor cells. Neuropilin-1 has a very short cytoplasmic domain and interacts with various coreceptors to form ligand-binding, signal-transducing receptor complexes. CD304 complexes with Plexin-A family members to bind chemorepellent Class 3 Semaphorins and guide neuronal axon growth. It also functions as a coreceptor with VEGFR2/CD309 to stimulate angiogenesis in response to VEGF165. CD304 mediates the interactions between some T cells and dendritic cells.

              

      566048 Rev. 1
      Format Details
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      Alexa Fluor™ 647
      Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 647
      Red 627-640 nm
      653 nm
      669 nm
      566048 Rev.1
      Citations & References
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      View product citations for antibody "566048" on CiteAb

      Development References (6)

      1. Bruder D et al. Neuropilin-1: a surface marker of regulatory T cells.. Eur J Immunol. 2004; 34(3):623-630. (Biology). View Reference
      2. Dzionek A et al. BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood.. J Immunol. 2000; 165(11):6037-6046. (Biology). View Reference
      3. Mizui M, Kumanogoh A, Kikutani H. Immune semaphorins: novel features of neural guidance molecules.. J Clin Immunol. 2009; 29(1):1-11. (Biology). View Reference
      4. Pan Q et al. Neuropilin-1 binds to VEGF121 and regulates endothelial cell migration and sprouting.. J Biol Chem. 2007; 282(33):24049-24056. (Biology). View Reference
      5. Renand A, Milpied P, Rossignol J, et al. Neuropilin-1 expression characterizes T follicular helper (Tfh) cells activated during B cell differentiation in human secondary lymphoid organs.. PLoS ONE. 2013; 8(12):e85589. (Biology). View Reference
      6. Soker S, Takashima S, Miao HQ, Neufeld G, Klagsbrun M. Neuropilin-1 is expressed by endothelial and tumor cells as an isoform-specific receptor for vascular endothelial growth factor.. Cell. 1998; 92(6):735-45. (Biology). View Reference
      View All (6) View Less
      566048 Rev. 1

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.