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Preparation And Storage
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
Companion Products
The PaT3 monoclonal antibody specifically binds to the Toll-like receptor 3 (TLR3), which is also known as CD283. CD283 belongs to the mammalian toll-like family of pattern recognition receptors (TLR) which participate in innate immune responses to microbial pathogens. It is a type I transmembrane glycoprotein that has an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the cytoplasmic region of IL-1RI/CD121a. CD283 is differentially expressed on splenic CD8+ dendritic cells, macrophages, and marginal zone B cells. It is expressed on the cell surface as well as intracellular compartments including endolysosomes. As in the case of viral infection, CD283 senses double-stranded RNA (dsRNA) in endolysosomes which subsequently leads to the production of antimicrobial peptides and proinflammatory cytokines by responding cells. CD283 can also be triggered by the synthetic analog, polyinosinic:polycytidylic acid (Poly I:C). The PaT3 monoclonal antibody can reportedly augment TLR3-mediated cellular responses to dsRNA. The PaT3 antibody does not crossreact with human CD283 (TLR3).
Development References (3)
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Alexopoulou L, Holt AC, Medzhitov R, Flavell RA. Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3.. Nature. 2001; 413(6857):732-8. (Biology). View Reference
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Jelinek I, Leonard JN, Price GE, et al. TLR3-specific double-stranded RNA oligonucleotide adjuvants induce dendritic cell cross-presentation, CTL responses, and antiviral protection.. J Immunol. 2011; 186(4):2422-9. (Biology). View Reference
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Murakami Y, Fukui R, Motoi Y, et al. Roles of the cleaved N-terminal TLR3 fragment and cell surface TLR3 in double-stranded RNA sensing.. J Immunol. 2014; 193(10):5208-17. (Immunogen: Blocking, Flow cytometry, Functional assay, Immunoprecipitation). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.