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      BV711 Rat Anti-Mouse IgG3
      BV711 Rat Anti-Mouse IgG3
      Flow cytometric analysis of CD3 expression on rat splenic leucocytes. Rat splenic leucocytes were preincubated with Purified Mouse Anti-Rat CD32 antibody (Rat BD Fc Block™) (Cat. No. 550270/550271). The cells were then either not incubated (background control staining; dashed line histogram) or incubated with Purified Mouse IgG3, κ Anti-Rat CD3 antibody (Cat. No. 554830/559974; solid line histogram). The cells were washed and then stained with BD Horizon™ BV711 Rat Anti-Mouse IgG3 antibody (Cat. No. 565809).  The fluorescence histogram showing CD3 expression (or background control staining) was derived from gated events with the forward and side light-scattering characteristics of viable leucocytes. Flow cytometric analysis was performed on a BD LSRFortessa™ Cell Analyzer System.
      BV711 Rat Anti-Mouse IgG3
      Flow cytometric analysis of CD3 expression on rat splenic leucocytes. Rat splenic leucocytes were preincubated with Purified Mouse Anti-Rat CD32 antibody (Rat BD Fc Block™) (Cat. No. 550270/550271). The cells were then either not incubated (background control staining; dashed line histogram) or incubated with Purified Mouse IgG3, κ Anti-Rat CD3 antibody (Cat. No. 554830/559974; solid line histogram). The cells were washed and then stained with BD Horizon™ BV711 Rat Anti-Mouse IgG3 antibody (Cat. No. 565809).  The fluorescence histogram showing CD3 expression (or background control staining) was derived from gated events with the forward and side light-scattering characteristics of viable leucocytes. Flow cytometric analysis was performed on a BD LSRFortessa™ Cell Analyzer System.
      Product Details
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      BD Horizon™
      Ighg3; Immunoglobulin heavy constant gamma 3
      Mouse (QC Testing)
      Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
      Mouse Pooled Ig
      Flow cytometry (Routinely Tested), Intracellular staining (flow cytometry) (Tested During Development)
      0.2 mg/ml
      AB_2739365
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV711 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV711 were removed.
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      Recommended Assay Procedures

      For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. BD Horizon Brilliant Violet 711 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
      8. Cy is a trademark of GE Healthcare.
      9. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
      10. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      565809 Rev. 2
      Antibody Details
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      R40-82

      The R40-82 antibody specifically recognizes mouse IgG3 of all strains. It does not react with other Ig isotypes.

      565809 Rev. 2
      Format Details
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      BV711
      The BD Horizon Brilliant Violet™ 711 (BV711) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 713-nm. BV711, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 710-nm (e.g., a 712/20-nm bandpass filter). The acceptor dye can be excited by the Red (628–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BV711
      Violet 405 nm
      407 nm
      713 nm
      565809 Rev.2
      Citations & References
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      View product citations for antibody "565809" on CiteAb

      Development References (2)

      1. Duong BH, Ota T, Aït-Azzouzene D, et al. Peripheral B cell tolerance and function in transgenic mice expressing an IgD superantigen.. J Immunol. 2010; 184(8):4143-58. (Clone-specific: ELISA, Flow cytometry). View Reference
      2. Kuzin II, Snyder JE, Ugine GD, et al. Tetracyclines inhibit activated B cell function.. Int Immunol. 2001; 13(7):921-31. (Clone-specific: Flow cytometry). View Reference
      565809 Rev. 2

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.