Skip to main content Skip to navigation
Hamburger Menu
Search icon
Order lookup icon Order lookup icon
Order Lookup
Country Selector Country Selector
United States (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      BV421 Rat Anti-Mouse CD281 (TLR1)
      Alert icon
      This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #
      BV421 Rat Anti-Mouse CD281 (TLR1)
      Analyses of CD281 (TLR1) Expression      Left and Middle Plots - Two-color flow cytometric analysis of mouse CD281 (TLR1) expression on mouse splenocytes. Splenic leucocytes were stained with Alexa Fluor® 647 Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 557683) and either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; Left Plot) or BD Horizon BV421 Rat Anti-Mouse CD281 (TLR1) antibody (Cat. No. 565792; Middle Plot). Two-color flow cytometric contour plots showing the correlated expression of CD45R/B220 versus CD281 (TLR1) [or Ig isotype control staining] expression were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.      Right Plot - Flow cytometric analysis of CD281 (TLR1) expression in mouse A20 cells. Cells from the mouse A20 (B lymphoma, ATCC TIB-208) cell line were stained with matching concentrations of either BV421 Rat IgG2a, κ Isotype Control (dashed line histogram) or BV421 Rat Anti-Mouse CD281 (TLR1) antibody (solid line histogram).  The fluorescence histogram showing CD281 (TLR1) [or Ig Isotype control staining] expression was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
      BV421 Rat Anti-Mouse CD281 (TLR1)
      Analyses of CD281 (TLR1) Expression      Left and Middle Plots - Two-color flow cytometric analysis of mouse CD281 (TLR1) expression on mouse splenocytes. Splenic leucocytes were stained with Alexa Fluor® 647 Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 557683) and either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; Left Plot) or BD Horizon BV421 Rat Anti-Mouse CD281 (TLR1) antibody (Cat. No. 565792; Middle Plot). Two-color flow cytometric contour plots showing the correlated expression of CD45R/B220 versus CD281 (TLR1) [or Ig isotype control staining] expression were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.      Right Plot - Flow cytometric analysis of CD281 (TLR1) expression in mouse A20 cells. Cells from the mouse A20 (B lymphoma, ATCC TIB-208) cell line were stained with matching concentrations of either BV421 Rat IgG2a, κ Isotype Control (dashed line histogram) or BV421 Rat Anti-Mouse CD281 (TLR1) antibody (solid line histogram).  The fluorescence histogram showing CD281 (TLR1) [or Ig Isotype control staining] expression was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
      Product Details
      Down Arrow Up Arrow


      BD Horizon™
      Cd281; Tlr1; Toll-like receptor 1; TIL; Toll/interleukin-1 receptor-like
      Mouse (QC Testing)
      Rat WI, also known as Wistar (outbred) IgG2a, κ
      Mouse TLR1 Transfected Cell Line
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      21897
      AB_2739357
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
      Show More blue down arrow Show Less blue up arrow

      Recommended Assay Procedures

      For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      6. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
      7. An isotype control should be used at the same concentration as the antibody of interest.
      8. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      Show More blue down arrow Show Less blue up arrow
      565792 Rev. 1
      Antibody Details
      Down Arrow Up Arrow
      TR23

      The TR23 monoclonal antibody specifically binds to CD281, which is also known as Toll-like receptor 1 (TLR1), or Toll/interleukin-1 receptor-like protein (TIL). CD281 is an ~98 kDa type I transmembrane glycoprotein that belongs to the Toll-like receptor (TLR) family. CD281 functions as a pattern recognition receptor (PRR) for pathogen-associated molecular patterns (PAMP) that are broadly expressed by microbial pathogens and plays essential roles in innate and adaptive immunity. In the process of binding microbial ligands, such as triacylated microbial lipopeptides, TLR1 associates with CD282 (TLR2). CD281 is expressed on B cells, monocytes/macrophages, and dendritic cells.  CD281/TLR1-deficient mice display impaired production of inflammatory cytokines in response to microbial ligands.

      565792 Rev. 1
      Format Details
      Down Arrow Up Arrow
      BV421
      The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BV421
      Violet 405 nm
      407 nm
      423 nm
      565792 Rev.1
      Citations & References
      Down Arrow Up Arrow
      View product citations for antibody "565792" on CiteAb

      Development References (5)

      1. Alexopoulou L, Thomas V, Schnare M et al. Hyporesponsiveness to vaccination with Borrelia burgdorferi OspA in humans and in TLR1- and TLR2-deficient mice. Nat Med. 8(8)(Biology). View Reference
      2. Miranda-Hernandez S, Gerlach N, Fletcher JM, et al. Role for MyD88, TLR2 and TLR9 but not TLR1, TLR4 or TLR6 in experimental autoimmune encephalomyelitis. J Immunol. 187(2)(Clone-specific: Flow cytometry). View Reference
      3. Motoi Y, Shibata T, Takahashi K, et al. Lipopeptides are signaled by Toll-like receptor 1, 2 and 6 in endolysosomes.. Int Immunol. 2014; 26(10):563-73. (Immunogen: Blocking, Flow cytometry, Immunoprecipitation). View Reference
      4. Pone EJ, Lou Z, Lam T et al. B cell TLR1/2, TLR4, TLR7 and TLR9 interact in induction of class switch DNA recombination: modulation by BCR and CD40, and relevance to T-independent antibody responses. Autoimmunity. 48(1)(Biology). View Reference
      5. Wyllie DH, Kiss-Toth E, Visintin A, et al. Evidence for an accessory protein function for Toll-like receptor 1 in anti-bacterial responses. J Immunol. 2000; 165(12):7125-7132. (Biology). View Reference
      View All (5) View Less
      565792 Rev. 1

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.