Flow cytometric analysis of fMLP receptor expression on human granulocytes. Human whole blood was stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 557714; dashed line histogram) or Alexa Fluor® 647 Mouse Anti-Human fMLP receptor antibody (Cat. No. 565623; solid line histogram). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The fluorescence histogram showing fMLP receptor expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact granulocytes. flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System
Flow cytometric analysis of fMLP receptor expression on human granulocytes. Human whole blood was stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Cat. No. 557714; dashed line histogram) or Alexa Fluor® 647 Mouse Anti-Human fMLP receptor antibody (Cat. No. 565623; solid line histogram). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The fluorescence histogram showing fMLP receptor expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact granulocytes. flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System
Product Details
BD Pharmingen™
fMet-Leu-Phe receptor; formyl peptide receptor 1; FPR1; FPR
Human (QC Testing)
Mouse C57BL/6 IgG1, κ
Human fMLP receptor Transfected Cell Line
Flow cytometry (Routinely Tested)
5 µl
AB_2739307
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Lysing Solution 10X Concentrate IVD
Size
100 mL
Cat No.
349202
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Alexa Fluor® 647 Mouse IgG1 κ Isotype Control RUO
Size
100 Tests
Cat No.
557714
565623 Rev. 1
Antibody Details
5F1
The 5F1 monoclonal antibody specifically binds to the N-formyl-leucyl-phenylalanine receptor (fMLP receptor), which is also known as, Formyl peptide receptor 1 (FPR1). This 55-70 kDa receptor is expressed on neutrophils, monocytes, macrophages, and liver parenchymal cells. It is composed of seven hydrophobic membrane spanning regions and is a member of the rhodopsin superfamily of G protein-coupled receptors. N-formyl peptides interact with fMLP receptors and induce neutrophil chemotaxis, phagocytosis, production of superoxide radicals and release of proteolytic enzymes. The binding of the 5F1 antibody to the fMLP receptor can be blocked by fMLP peptide.
565623 Rev. 1
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
565623 Rev.1
Citations & References
View product citations for antibody "565623" on CiteAb
Development References (4)
-
Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
-
Lala A, Sojar HT, De Nardin E. Expression and purification of recombinant human N-formyl-L-leucyl-L-phenylalanine (FMLP) receptor: generation of polyclonal antibody against FMLP receptor. Biochem Pharmacol. 1997; 54(3):381-390. (Biology). View Reference
-
Prossnitz ER, Gilbert TL, Chiang S, et al. Multiple activation steps of the N-formyl peptide receptor.. Biochemistry. 1999; 38(8):2240-7. (Immunogen). View Reference
-
Sengeløv H, Boulay F, Kjeldsen L, Borregaard N. Subcellular localization and translocation of the receptor for N-formylmethionyl-leucyl-phenylalanine in human neutrophils. Biochem J. 1994; 299(2):473-479. (Biology). View Reference
565623 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.