Flow cytometric analysis of Human Blood Group A expression on human red blood cells. Human red blood cells were stained with either Alexa Fluor® 647 Mouse IgG3, κ Isotype Control (Cat. No. 560803; dashed line histogram) or Alexa Fluor® 647 Mouse Anti-Human Blood Group A antibody (Cat. No. 565384; solid line histogram). The fluorescence histogram showing Human Blood Group A expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of erythrocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Flow cytometric analysis of Human Blood Group A expression on human red blood cells. Human red blood cells were stained with either Alexa Fluor® 647 Mouse IgG3, κ Isotype Control (Cat. No. 560803; dashed line histogram) or Alexa Fluor® 647 Mouse Anti-Human Blood Group A antibody (Cat. No. 565384; solid line histogram). The fluorescence histogram showing Human Blood Group A expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of erythrocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Pharmingen™
Human (QC Testing)
Mouse IgG3, κ
Flow cytometry (Routinely Tested)
5 µl
AB_2739210
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Alexa Fluor® 647 Mouse IgG3, κ Isotype Control RUO
Size
0.1 mg
Cat No.
560803
565384 Rev. 1
Antibody Details
NaM87-1F6
The NaM87-1F6 monoclonal antibody specifically binds to blood group A expressed on human erythrocytes. Clone NaM87-1F6 induces agglutination of human group A red blood cells. It does not react with human group B, or group O red blood cells.
565384 Rev. 1
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
565384 Rev.1
Citations & References
View product citations for antibody "565384" on CiteAb
Development References (3)
-
Blanchard D, Bruneau V, Bernard D, et al. Flow cytometry analysis of dual red blood cell populations after bone marrow transplantation. Br J Haematol. 1995; 89(4):741-747. (Clone-specific: Flow cytometry). View Reference
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David B, Bernard D, Navenot JM, Muller JY, Blanchard D. Flow cytometric monitoring of red blood cell chimerism after bone marrow transplantation. Transfus Med. 1999; 9(3):209-217. (Clone-specific: Flow cytometry).
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Walker RH, ed. Technical Manual of the American Association of Blood Banks. Arlington VA: 1990.
565384 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.