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      APC-R700 Rat Anti-Mouse Siglec-F
      APC-R700 Rat Anti-Mouse Siglec-F
      Two-color flow cytometric analysis of CD170 (Siglec-F) expression on mouse bone marrow cells. Bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with FITC Rat Anti-Mouse CD11b antibody (Cat. No. 553310/557396/561688) and with either BD Horizon™ APC-R700 Rat IgG2a, κ Isotype Control (Cat. No. 564982; Left Plot) or BD Horizon APC-R700 Rat Anti-Mouse CD170 (Siglec-F) antibody (Cat. No. 565183; Right Plot). Two-color flow cytometric dot plots showing the correlated expression of CD170 (Siglec-F) [or Ig Isotype control staining] versus CD11b were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System. For optimal flow cytometric analysis, we suggest that this reagent be titrated starting at less than or equal to 0.25 µg per million cells in a 100 µl volume.
      APC-R700 Rat Anti-Mouse Siglec-F
      Two-color flow cytometric analysis of CD170 (Siglec-F) expression on mouse bone marrow cells. Bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with FITC Rat Anti-Mouse CD11b antibody (Cat. No. 553310/557396/561688) and with either BD Horizon™ APC-R700 Rat IgG2a, κ Isotype Control (Cat. No. 564982; Left Plot) or BD Horizon APC-R700 Rat Anti-Mouse CD170 (Siglec-F) antibody (Cat. No. 565183; Right Plot). Two-color flow cytometric dot plots showing the correlated expression of CD170 (Siglec-F) [or Ig Isotype control staining] versus CD11b were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System. For optimal flow cytometric analysis, we suggest that this reagent be titrated starting at less than or equal to 0.25 µg per million cells in a 100 µl volume.
      Product Details
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      BD Horizon™
      CD170; SIGL5; Siglec-5; Siglec5; Siglecf; sialic acid-binding Ig-like lectin 5; siglec-5
      Mouse (QC Testing)
      Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
      Mouse Siglec-F and human IgG Fc recombinant fusion protein
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      AB_2739097
      Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon APC-R700 under optimum conditions, and unconjugated antibody and free BD Horizon APC-R700 were removed.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. An isotype control should be used at the same concentration as the antibody of interest.
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      565183 Rev. 2
      Antibody Details
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      E50-2440

      The E50-2440 monoclonal antibody specifically recognizes Siglec-F. Siglecs are the sialic acid-binding immunoglobulin superfamily lectins defined in the human, each of which has a distinctive expression pattern in the hematopoietic system and at least some of which are known to mediate cell-cell interactions. Orthologous proteins of human Siglec-1 (Sialoadhesin or CD169), Siglec-2 (CD22), and Siglec-4 (myelin-associated glycoprotein) have been characterized in the mouse. Human Siglec-3 (CD33) and Siglecs-5 through -10 are encoded by a cluster of closely related genes, and each has two cytoplasmic ITIM (Immunoreceptor Tyrosine-based Inhibitory Motifs). Similarly, mouse Siglec-F is encoded by the Siglecf gene in a syntenic cluster in the mouse, and the protein has sialic acid-binding activity and an intracytoplasmic ITIM. Its expression pattern differs from those of the human Siglec-3-related proteins in that it is found on immature cells of the myelomonocytic lineage, with reduced expression on mature neutrophils and monocytes, and not on lymphoid cells. It has been proposed that mAb E50-2440 may be used for identification of immature myelomonocytic cells in the mouse.

      This antibody was conjugated to BD Horizon APC-R700, which has been developed exclusively by BD Biosciences as a better alternative to Alexa Fluor® 700. APC-R700 excites and emits at similar wavelengths to Alexa Fluor® 700 yet exhibits significantly improved brightness. This dye can be excited by the red laser and detected with the same filter set as Alexa Fluor® (eg, 730/45-nm filter).

      565183 Rev. 2
      Format Details
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      APC-R700
      The BD Horizon™ APC-R700 (APC-R700) Dye is a part of the BD APC red family of dyes. This tandem fluorochrome is comprised of an Allophycocyanin (APC) dye donor that has excitation maximum (Ex Max) of 651-nm and an acceptor dye, R700, with an emission maximum (Em Max) at 706-nm. APC-R700, driven by BD innovation, is designed to be excited by the red (627–640-nm) laser and detected using an optical filter centered near 710-nm (e.g., a 720/40-nm bandpass filter). APC-R700 is a brighter alternative to Alexa Fluor™ 700. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      APC-R700
      Red 627-640 nm
      651 nm
      706 nm
      565183 Rev.2
      Citations & References
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      View product citations for antibody "565183" on CiteAb

      Development References (2)

      1. Angata T, Hingorani R, Varki NM, Varki A. Cloning and characterization of a novel mouse Siglec, mSiglec-F: differential evolution of the mouse and human (CD33) Siglec-3-related gene clusters. J Biol Chem. 2001; 276(48):45128-45136. (Immunogen: Flow cytometry, Fluorescence activated cell sorting). View Reference
      2. Crocker PR, Varki A. Siglecs, sialic acids and innate immunity. Trends Immunol. 2001; 22(6):337-342. (Biology). View Reference
      565183 Rev. 2

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.