PE Rat Anti-Mouse CD107b

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BD Pharmingen™ PE Rat Anti-Mouse CD107b

Name: PE Rat Anti-Mouse CD107b
Brand: BD Pharmingen™
SKU: 564246

Clone ABL-93

(RUO)

Flow cytometric analysis of CD107b expression in mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and washed and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either PE Rat IgG2a, λ Isotype Control (Cat. No. 557076; dashed line histogram) or PE Rat Anti-Mouse CD107b antibody (Cat. No. 564246; solid line histogram). The fluorescence histogram showing CD107b expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.

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Product Details

Brand: BD Pharmingen™

Alternative Name: Lamp2; LAMP-2; LAMP II; Lysosomal-associated membrane protein 2; LGP-B

Reactivity: Mouse (QC Testing)

Isotype: Rat SD, also known as Sprague-Dawley (outbred) IgG2a, λ

Immunogen: Glycoproteins purified from BALB/c mouse embryo 3T3 cell line

Application: Intracellular staining (flow cytometry) (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_2738699

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO

Size 0.1 mg Cat No. 553141

Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO

Size 0.5 mg Cat No. 553142

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Lysing Buffer RUO

Size 100 mL Cat No. 555899

Fixation Buffer RUO

Size 100 mL Cat No. 554655

View Details

564246 Rev. 1

Antibody Details

ABL-93

The ABL-93 monoclonal antibody specifically recognizes CD107b which is also known as Lysosome-associated membrane protein 2 (LAMP2/LAMP-2), Lysosomal membrane glycoprotein type B (LGP-B), and Mac-3. CD107b is a type I lysosomal transmembrane glycoprotein that serves as a useful marker to distinguish lysosomes from other cellular organelles. Mouse CD107b consists of a ~40-kDa core protein which is heavily glycosylated to form heterogeneous mature glycoproteins of 110-120 kDa. It is principally expressed in epithelial cells and macrophages in a variety of organs in normal and Beige mutant mice.

564246 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

564246 Rev.1

Citations & References

View product citations for antibody "564246" on CiteAb

Development References (3)

Cha Y, Holland SM, August JT. The cDNA sequence of mouse LAMP-2. Evidence for two classes of lysosomal membrane glycoproteins. J Biol Chem. 1990; 265(9):5008-5013. (Biology). View Reference
Chen JW, Chen GL, D'Souza MP, Murphy TL, August JT. Lysosomal membrane glycoproteins: properties of LAMP-1 and LAMP-2. Biochem Soc Symp. 1986; 51:97-112. (Clone-specific: Fluorescence microscopy, Immunofluorescence). View Reference
Chen JW, Murphy TL, Willingham MC, Pastan I, August JT. Identification of two lysosomal membrane glycoproteins. J Cell Biol. 1985; 101(1):85-95. (Immunogen: Fluorescence microscopy, Immunofluorescence, Immunoprecipitation, Radioimmunoassay). View Reference

564246 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.