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      BV786 Rat Anti-Mouse IgM
      BV786 Rat Anti-Mouse IgM
      Two-color flow cytometric analysis of IgM expression on mouse splenocytes. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with PE-CF594 Hamster Anti-Mouse CD3e (Cat. No. 562286/562332) and either BV786 Rat IgG2a, κ Isotype Control (Cat. No. 563335; Left Panel) or BV786 Rat Anti-Mouse IgM (Cat. No. 564028, Right Panel). The fluorescence two-color dot plots were derived from gated events based on the light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD™ LSR II.
      BV786 Rat Anti-Mouse IgM
      Two-color flow cytometric analysis of IgM expression on mouse splenocytes. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with PE-CF594 Hamster Anti-Mouse CD3e (Cat. No. 562286/562332) and either BV786 Rat IgG2a, κ Isotype Control (Cat. No. 563335; Left Panel) or BV786 Rat Anti-Mouse IgM (Cat. No. 564028, Right Panel). The fluorescence two-color dot plots were derived from gated events based on the light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD™ LSR II.
      Product Details
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      BD Horizon™
      Ighm; Igh-M; Immunoglobulin M; Igh6; muH; immunoglobulin heavy constant mu
      Mouse (QC Testing)
      Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
      Pooled Mouse Ig
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      AB_2738553
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV786 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV786 were removed.
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      Recommended Assay Procedures

      For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Cy is a trademark of GE Healthcare.
      7. BD Horizon Brilliant Violet 786 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
      8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
      9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      564028 Rev. 2
      Antibody Details
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      R6-60.2

      The R6-60.2 antibody monoclonal antibody specifically binds to mouse Immunoglobulin M (IgM) of Igh-C[a] and Igh-C[b] haplotypes. It does not react with other Ig isotypes. The R6-60.2 antibody has not been shown to stimulate B-cell proliferation.

      564028 Rev. 2
      Format Details
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      BV786
      The BD Horizon Brilliant Violet™ 786 (BV786) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an Ex Max of 407-nm and an acceptor dye with an Em Max at 786-nm.  BV786, driven by BD innovation, is designed to be excited by the violet laser and detected using a filter, centered near 785 nm (e.g. 780/60 nm bandpass filter).  Please ensure that your instrument’s configurations (lasers and filters) are appropriate for this dye.
      altImg
      BV786
      Violet 405 nm
      407 nm
      786 nm
      564028 Rev.2
      Citations & References
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      View product citations for antibody "564028" on CiteAb

      Development References (3)

      1. BD Biosciences Pharmingen. Unpublished results. .
      2. Gavin AL, Duong B, Skog P, et al. δBAFF, a splice isoform of BAFF, opposes full-length BAFF activity in vivo in transgenic mouse models. J Immunol. 2005; 75(1):319-328. (Clone-specific: ELISA). View Reference
      3. Touma M, Keskin DB, Shiroki F, et al. Impaired B cell development and function in the absence of IκBNS. J Immunol. 2011; 187(8):3942-3952. (Clone-specific: Flow cytometry). View Reference
      564028 Rev. 2

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.