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      BUV395 Mouse Anti-Human TRA-1-60 Antigen
      BUV395 Mouse Anti-Human TRA-1-60 Antigen
      Flow cytometric analysis of BD HorizonTM BUV395 anti-TRA-1-60 on human embryonic stem cells. H9 human embryonic stem (ES) cells (WiCell, Madison, WI) passage 44 grown in mTESR™1 media (StemCell Technologies) were harvested with Accutase™ (Cat. No. 561527) and stained with either BD Horizon™ BUV395 anti-TRA-1-60 (solid line) or BD Horizon™ BUV395 mouse IgM, isotype control (Clone G155-228, Cat. No.563866, dashed line). Flow cytometry was performed on a BD LSR™ II flow cytometry system.
      BUV395 Mouse Anti-Human TRA-1-60 Antigen
      Flow cytometric analysis of BD HorizonTM BUV395 anti-TRA-1-60 on human embryonic stem cells. H9 human embryonic stem (ES) cells (WiCell, Madison, WI) passage 44 grown in mTESR™1 media (StemCell Technologies) were harvested with Accutase™ (Cat. No. 561527) and stained with either BD Horizon™ BUV395 anti-TRA-1-60 (solid line) or BD Horizon™ BUV395 mouse IgM, isotype control (Clone G155-228, Cat. No.563866, dashed line). Flow cytometry was performed on a BD LSR™ II flow cytometry system.
      Product Details
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      BD Horizon™
      TRA-1-60(R)
      Human (QC Testing), Rhesus, Monkey (Reported)
      Mouse BALB/c IgM, κ
      Human Embryonal Carcinoma Cell Line
      Flow cytometry (Routinely Tested)
      5 µl
      AB_2631259
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV395 under optimum conditions, and unconjugated antibody and free BD Horizon BUV395 were removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      5. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
      6. mTESR™1 is a trademark of StemCell Technologies.
      7. Accutase is a registered trademark of Innovative Cell Technologies, Inc.
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      563878 Rev. 1
      Antibody Details
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      TRA-1-60

      The TRA-1-60 monoclonal antibody reacts with the neuraminidase-resistant form of a pluripotent-stem-cell-specific epitope on a high-molecular-weight transmembrane glycoprotein.  The TRA-1-60 antigen is a sialylated epitope on the same keratan sulfate core molecule, podocalyxin, as 4 other distinct antigens on tumor-derived cell lines, TRA-1-81, GCTM2, K4, and K21.  The expression of TRA-1-60 antigen is stage-specific and can be used to characterize embryonic cells and monitor their differentiation.  The antigen is found on teratocarcinoma (embryonal carcinoma or EC), embryonic inner cell mass (but not morula or trophoblast), and embryonic stem (ES) cells.  TRA-1-60 antigen is released into the serum of patients bearing  testicular tumors containing EC cells.  As human EC and ES cells undergo differentiation, expression of TRA-1-60 antigen is lost.  Expression of TRA-1-60 antigen has also been observed on a rhesus monkey ES cell line (Thomson et al, 1995).

      The antibody was conjugated to BD Horizon™ BUV395 which has been exclusively developed by BD Biosciences as an optimal dye for use on a 355 nm laser equipped instrument. With an Ex Max at 348 nm  and an Em Max at 395 nm, this dye has virtually no spillover into any other detector. BD Horizon™ BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter.

      563878 Rev. 1
      Format Details
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      BUV395
      The BD Horizon Brilliant™ Ultraviolet 395 (BUV395) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This base dye is a polymer fluorochrome with an excitation maximum (Ex Max) of 348-nm and an emission maximum (Em Max) at 395-nm. Driven by BD innovation, BUV395 is designed to be excited by the ultraviolet laser (355-nm) and detected using an optical filter centered near 380-nm (e.g., 379/28-nm bandpass filter). BUV395 is the ideal dye when using only one detector on the ultraviolet laser as it spills into no other detectors and no other fluors spill into it. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BUV395
      Ultraviolet 355 nm
      348 nm
      395 nm
      563878 Rev.1
      Citations & References
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      View product citations for antibody "563878" on CiteAb

      Development References (7)

      1. Andrews PW, Banting G, Damanov I, Arnaud D, Avner P. Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma. 1984; 3(4):347-361. (Immunogen). View Reference
      2. Badcock G, Pigott C, Goepel J, Andrews PW. The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan. Cancer Res. 1999; 59:4715-4719. (Clone-specific). View Reference
      3. Draper JS, Pigott C, Thomson JA, Andrews PW. Surface antigens of human embryonic stem cells: changes upon differentiation in culture. J Anat. 2002; 200:249-258. (Clone-specific). View Reference
      4. Henderson JK, Draper JS, Baillie HS, et al. Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells. 2002; 20:329-337. (Clone-specific). View Reference
      5. Schopperle WM, DeWolf WC. The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma. Stem Cells. 2007; 25:723-730. (Clone-specific). View Reference
      6. Thomson JA, Itskovitz-Eldor J, Shapiro SS, et al. Embryonic stem cell lines derived from human blastocysts. Science. 1998; 282:1145-1147. (Clone-specific). View Reference
      7. Thomson JA, Kalishman J, Golos TG, et al. Isolation of a primate embryonic stem cell line. Proc Natl Acad Sci U S A. 1995; 92:7844-7848. (Clone-specific). View Reference
      View All (7) View Less
      563878 Rev. 1

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.