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Flow cytometric analysis of EGF Receptor expression on human A-431 cells. Cells from the human A-431 epidermoid carcinoma cell Line (ATCC CRL-1555) were stained with Alexa Fluor® 647 Mouse Anti-Human EGF Receptor antibody (Cat. No. 563577; solid line histogram) or with Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (Cat. No. 557903; dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Human EGF Receptor
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Companion Products
The EGFR.1 monoclonal antibody specifically binds to the epidermal growth factor receptor (EGF-R). The EGF-R is a transmembrane glycoprotein of approximately 170 kDa that is expressed on most cells. It consists of a glycosylated extracellular domain which binds to epidermal growth factor (EGF), a transmembrane domain, and an intracellular domain with tyrosine-kinase activity essential for signal transduction. The EGF-R plays an important role in the growth and differentiation of many cellular types. Transforming growth factor α (TGFα), vaccinia virus growth factor, and related growth factors can also bind to and signal through the EGF-R.
Development References (4)
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Fernandez A, Spitzer E, Perez R. A new monoclonal antibody for detection of EGF-receptors in western blots and paraffin-embedded tissue sections. J Cell Biol. 1992; 49(2):157-165. (Biology). View Reference
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Fitzgerald KA, Callard RE. The cytokine factsbook., 2nd ed. / Katherine A. Fitzgerald ... [et al.].. San Diego: Academic Press; 2001:1-515.
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Mishima K, Johns TG, Luwor RB, et al. Growth suppression of intracranial xenografted glioblastomas overexpressing mutant epidermal growth factor receptors by systemic administration of monoclonal antibody (mAb) 806, a novel monoclonal antibody directed to the receptor. Cancer Res. 2001; 61(14):5349-5354. (Clone-specific: Flow cytometry, Immunoprecipitation). View Reference
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Troalen F, Maudelondé T, Montcourrier P, Bohuon C, Delarue JC. Characterization of two monoclonal antibodies against the COOH-terminal part of the human epidermal growth factor receptor and potential clinical use. J Immunol Methods. 1991; 143(2):175-186. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.