Flow cytometric analysis of human CD371 (Clec12A) expression on monocytes and dendritic cell subsets from human PBMC. Human peripheral blood mononuclear cells (PBMC) were stained with either PE Mouse IgG2a, κ Isotype Control (Cat. No. 554648) or PE Mouse Anti-Human CD371 (Clec12A) (Cat. No. 562566) antibody. The cells were also stained with PE-Cy™7 Mouse Anti-Human HLA-DR (Cat. No. 560651), Alexa Fluor® 700 Mouse Anti-Human CD11c (Cat. No. 561352), PE-Cy™5 Mouse Anti-Human CD123 (Cat. No. 551065), BD Horizon™ PE-CF594 Mouse Anti-Human CD14 (Cat. No. 562334) and a lineage cocktail comprised of BD Horizon™ V450 Mouse Anti-Human CD3 (Cat. No. 560366), CD14 (Cat. No. 560350), CD19 (Cat. No. 560354) and CD56 (Cat. No. 560361). Histograms showing the expression of CD371 (solid line) or Ig isotype control staining (dotted line) on CD14+ monocytes (Left Panel), Lineage-HLA-DR+CD11c+ myeloid dendritic cells (Middle Panel) and Lineage-HLA-DR+CD123+ plasmacytoid dendritic cells (Right Panel) were derived from gated events with the forward and side light-scatter characteristics of viable monocytes or lymphocytes (for dendritic cells), respectively. Flow cytometry was performed using a BD™ LSRII Flow Cytometer System.
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Flow cytometric analysis of human CD371 (Clec12A) expression on monocytes and dendritic cell subsets from human PBMC. Human peripheral blood mononuclear cells (PBMC) were stained with either PE Mouse IgG2a, κ Isotype Control (Cat. No. 554648) or PE Mouse Anti-Human CD371 (Clec12A) (Cat. No. 562566) antibody. The cells were also stained with PE-Cy™7 Mouse Anti-Human HLA-DR (Cat. No. 560651), Alexa Fluor® 700 Mouse Anti-Human CD11c (Cat. No. 561352), PE-Cy™5 Mouse Anti-Human CD123 (Cat. No. 551065), BD Horizon™ PE-CF594 Mouse Anti-Human CD14 (Cat. No. 562334) and a lineage cocktail comprised of BD Horizon™ V450 Mouse Anti-Human CD3 (Cat. No. 560366), CD14 (Cat. No. 560350), CD19 (Cat. No. 560354) and CD56 (Cat. No. 560361). Histograms showing the expression of CD371 (solid line) or Ig isotype control staining (dotted line) on CD14+ monocytes (Left Panel), Lineage-HLA-DR+CD11c+ myeloid dendritic cells (Middle Panel) and Lineage-HLA-DR+CD123+ plasmacytoid dendritic cells (Right Panel) were derived from gated events with the forward and side light-scatter characteristics of viable monocytes or lymphocytes (for dendritic cells), respectively. Flow cytometry was performed using a BD™ LSRII Flow Cytometer System.
Product Details
BD Pharmingen™
CD371; Clec12A; MICL; CLL-1; DCAL-2
Human (QC Testing)
Mouse BALB/c IgG2a, κ
Human CLEC12A Transfected Cell Line
Flow cytometry (Routinely Tested)
5 µl
X 10-73
AB_11154581
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- CF™ is a trademark of Biotium, Inc.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Cy is a trademark of GE Healthcare.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
PE Mouse IgG2a, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554648
PE-Cy™5 Mouse Anti-Human CD123 RUO
Size
100 Tests
Cat No.
551065
PE-CF594 Mouse Anti-Human CD14 RUO
Size
25 Tests
Cat No.
562334
V450 Mouse Anti-Human CD3 RUO
Size
30 Tests
Cat No.
560366
V450 Mouse Anti-Human CD14 RUO
Size
30 Tests
Cat No.
560350
562566 Rev. 3
Antibody Details
50C1
The 50C1 monoclonal antibody specifically binds to human CD371 which is also known as Clec12A (C-type lectin domain family 12 member A), C-type lectin-like molecule 1 (CLL-1), myeloid inhibitory C-type lectin-like receptor (MICL), or dendritic cell-associated lectin 2 (DCAL-2). It is expressed on a variety of cells including monocytes, macrophages, dendritic cells, and granulocytes and perhaps some NK cells. Clec12A is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. It is a 30 kDa type II transmembrane glycoprotein that has one single C-type lectin-like domain and one cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). Clec12A has similarity with the β-glucan receptor (Dectin-1) and LOX-1 with high N-glycosylation. There are at least five isoforms due to alternative transcript splicing. Signaling through Clec12A can induce internalization of Clec12A, dendritic cell maturation and the production of cytokines including IL-12. Clec12A may also serve as a negative regulator of activated leukocytes recruited to sites of inflammation.
562566 Rev. 3
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 561 nm
496 nm, 566 nm
576 nm
562566 Rev.3
Citations & References
View product citations for antibody "562566" on CiteAb
Development References (5)
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Chen CH, Floyd H, Olson NE, et al. Dendritic-cell-associated C-type lectin 2 (DCAL-2) alters dendritic-cell maturation and cytokine production. Blood. 2006; 107(4):1459-1467. (Biology). View Reference
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Lahoud MH, Proietto AI, Ahmet F, et al. The C-type lectin Clec12A present on mouse and human dendritic cells can serve as a target for antigen delivery and enhancement of antibody responses.. J Immunol. 2009; 182(12):7587-94. (Immunogen: Flow cytometry). View Reference
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Marshall AS, Willment JA, Lin HH, Williams DL, Gordon S, Brown GD. Identification and characterization of a novel human myeloid inhibitory C-type lectin-like receptor (MICL) that is predominantly expressed on granulocytes and monocytes.. J Biol Chem. 2004; 279(15):14792-802. (Biology). View Reference
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Marshall AS, Willment JA, Pyz E, et al. Human MICL (CLEC12A) is differentially glycosylated and is down-regulated following cellular activation.. Eur J Immunol. 2006; 36(8):2159-69. (Biology). View Reference
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van Rhenen A, van Dongen GA, Kelder A, et al. The novel AML stem cell associated antigen CLL-1 aids in discrimination between normal and leukemic stem cells.. Blood. 2007; 110(7):2659-66. (Biology). View Reference
562566 Rev. 3
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.