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      BV421 Mouse Anti-Human CD4
      BV421 Mouse Anti-Human CD4
      Flow cytometric analysis of CD4 expression on human peripheral blood lymphocytes. Human whole blood was stained at 4°C with the BD Horizon™ BV421 Mouse anti-Human CD4 antibody (Cat. No. 562424/562425; solid line histogram) or with a BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histogram). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD FACSCanto™ II Flow Cytometer System.
      BV421 Mouse Anti-Human CD4
      Immunohistofluorescent analysis of CD4 expression by cells within human tonsil. A human tonsil cryosection (5 µm) was fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), blocked with 5% goat serum and 1% BSA diluted in 1x PBS, and stained with BD Pharmingen™ Purified Mouse Anti-Human CD8 antibody (Cat. No. 555364) followed by BD Horizon™ BV480 Goat Anti-Mouse Ig second step antibody (Cat. No. 564877, pseudo-colored green). Sections were thoroughly washed, then stained with BD Horizon™ BV421 Mouse Anti-Human CD4 antibody (Cat. No. 562424/562425, pseudo-colored red) and Alexa Fluor® 488 Mouse Anti-Human CD19 antibody (Cat. No. 557697, pseudo-colored blue). Images were captured on a standard epifluorescence microscope. Original magnification, 20x.
      BV421 Mouse Anti-Human CD4 BV421 Mouse Anti-Human CD4
      Flow cytometric analysis of CD4 expression on human peripheral blood lymphocytes. Human whole blood was stained at 4°C with the BD Horizon™ BV421 Mouse anti-Human CD4 antibody (Cat. No. 562424/562425; solid line histogram) or with a BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histogram). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD FACSCanto™ II Flow Cytometer System.
      Immunohistofluorescent analysis of CD4 expression by cells within human tonsil. A human tonsil cryosection (5 µm) was fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), blocked with 5% goat serum and 1% BSA diluted in 1x PBS, and stained with BD Pharmingen™ Purified Mouse Anti-Human CD8 antibody (Cat. No. 555364) followed by BD Horizon™ BV480 Goat Anti-Mouse Ig second step antibody (Cat. No. 564877, pseudo-colored green). Sections were thoroughly washed, then stained with BD Horizon™ BV421 Mouse Anti-Human CD4 antibody (Cat. No. 562424/562425, pseudo-colored red) and Alexa Fluor® 488 Mouse Anti-Human CD19 antibody (Cat. No. 557697, pseudo-colored blue). Images were captured on a standard epifluorescence microscope. Original magnification, 20x.
      Product Details
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      BD Horizon™
      L3T4; T-cell surface antigen T4/Leu-3; W3/25; CD4 antigen (p55)
      Human (QC Testing)
      Mouse IgG1, κ
      PHA-stimulated Human Peripheral Blood Mononuclear Cells
      Flow cytometry (Routinely Tested), Immunofluorescence (Reported)
      5 µl
      IV T114; VI 6T-079
      AB_11154417
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
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      Recommended Assay Procedures

      For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
      8. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
      9. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      10. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      562425 Rev. 3
      Antibody Details
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      RPA-T4

      The RPA-T4 monoclonal antibody specifically binds to CD4, a 59 kDa single-chain transmembrane glycoprotein that is expressed on T-helper/inducer cell populations. CD4 is also expressed on thymocyte subsets and at lower levels on monocytes and macrophages. CD4 functions as a co-receptor in MHC class II-restricted antigen-induced T cell activation and as a receptor for human immunodeficiency viruses (HIV). This antibody binds to the D1 domain (CDR1 and CDR3 epitopes) of the CD4 antigen and reacts with approximately 80% of thymocytes and 45% of peripheral blood lymphocytes. RPA-T4 is capable of blocking HIV-1, gp120, and inhibits syncytium formation.

      The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes.  With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421  can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon™ BV421  conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.

      562425 Rev. 3
      Format Details
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      BV421
      The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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      BV421
      Violet 405 nm
      407 nm
      423 nm
      562425 Rev.3
      Citations & References
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      View product citations for antibody "562425" on CiteAb

      Development References (3)

      1. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
      2. Piatier-Tonneau D. CD4 workshop panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:49-54.
      3. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
      562425 Rev. 3

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.