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      FITC Rat Anti-Mouse CD3 Molecular Complex
      FITC Rat Anti-Mouse CD3 Molecular Complex
      CD3 expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with FITC-conjugated 17A2 (Cat. No. 555274) and PE-conjugated anti-mouse CD19 mAb 1D3 (Cat. No. 557399, left panel). BALB/c thymocytes were also stained with FITC-conjugated 17A2 mAb (Cat. No. 555274, right panel). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
      FITC Rat Anti-Mouse CD3 Molecular Complex
      CD3 expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with FITC-conjugated 17A2 (Cat. No. 555274) and PE-conjugated anti-mouse CD19 mAb 1D3 (Cat. No. 557399, left panel). BALB/c thymocytes were also stained with FITC-conjugated 17A2 mAb (Cat. No. 555274, right panel). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
      Product Details
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      BD Pharmingen™
      CD3; CD3 epsilon; Cd3e; CD3ε; T3e
      Mouse (QC Testing)
      Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ
      γδ TCR-positive T-T hybridoma D1
      Flow cytometry (Routinely Tested)
      0.5 mg/ml
      12501
      AB_395698
      Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      561798 Rev. 2
      Antibody Details
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      17A2

      The 17A2 monoclonal antibody specifically binds to the T-cell receptor-associated CD3 complex that is expressed on many thymocytes and mature T lymphocytes. Plate-bound 17A2 antibody has been reported to induce IL-2 production by cultured T cells in the absence of accessory cells. The binding of the 17A2 antibody to T cells can be blocked by the anti-CD3e mAb 145-2C11 (Cat. No. 557306/553058/550275). This suggests that the 17A2 antibody recognizes an epitope of the CD3 epsilon chain. In vivo treatment with 17A2 antibody has been reported to partially deplete T lymphocytes and temporarily down-modulates CD3 expression on T cells.

      561798 Rev. 2
      Format Details
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      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      561798 Rev.2
      Citations & References
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      View product citations for antibody "561798" on CiteAb

      Development References (2)

      1. Miescher GC, Schreyer M, MacDonald HR. Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex. Immunol Lett. 1989; 23(2):113-118. (Immunogen: Cytotoxicity, Functional assay, Immunohistochemistry, Immunoprecipitation, Stimulation). View Reference
      2. Mysliwietz J, Thierfelder S. Antilymphocytic antibodies and marrow transplantation. XII. Suppression of graft-versus-host disease by T-cell-modulating and depleting antimouse CD3 antibody is most effective when preinjected in the marrow recipient. Blood. 1992; 80(10):2661-2667. (Clone-specific: Depletion). View Reference
      561798 Rev. 2

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.