Alexa Fluor® 647 Mouse Anti-Pig CD29

BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Pig CD29

Name: Alexa Fluor® 647 Mouse Anti-Pig CD29
Brand: BD Pharmingen™
SKU: 561496

Clone NaM160-1A3

(RUO)

Flow cytometric analysis of CD29 expression on pig peripheral blood leukocytes. Pig whole blood was stained with either Alexa Fluor® 647 Mouse Anti-Pig CD29 antibody (Cat. No. 561496, solid line histogram) or with an Alexa Fluor® 647 mouse IgG1, κ Isotype Control (Cat. No. 557714; dashed line histogram). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes (Left Panel), monocytes (Middle Panel) or granulocytes (Right Panel). Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

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Product Details

Brand: BD Pharmingen™

Alternative Name: Integrin β1 chain

Reactivity: Pig (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Pig platelets

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_10895582

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Lysing Buffer RUO

Size 100 mL Cat No. 555899

Alexa Fluor® 647 Mouse IgG1 κ Isotype Control RUO

Size 100 Tests Cat No. 557714

561496 Rev. 1

Antibody Details

NaM160-1A3

The NaM160-1A3 monoclonal antibody specifically binds to the 116-kDa integrin β1 chain (CD29). CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin α chains. With α1 through α6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with αv (CD51), it forms αvβ1 integrin. As a result, CD29 has a broad tissue distribution, including leukocytes, endothelia, epithelia, and oocytes. Porcine CD29 is believed to be a major target for natural antibodies involved in rejection of pig-to-human xenografts, and a mAb to block recognition of pig CD29 may have therapeutic applications. NaM160-1A3 mAb does not crossreact with human peripheral blood lymphocytes or umbilical cord vein endothelial cells.

561496 Rev. 1

Format Details

Alexa Fluor™ 647

Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: Alexa Fluor™ 647

Excitation Source: Red 627-640 nm

Excitation Max: 653 nm

Emission Max: 669 nm

561496 Rev.1

Citations & References

View product citations for antibody "561496" on CiteAb

Development References (4)

Jiménez-Marín A, Garrido JJ, de Andrés-Cara DF, Morera L, Barbancho MJ, Llanes D. Molecular cloning and characterization of the pig homologue to human CD29, the integrin beta1 subunit. Transplantation. 2000; 70(4):649-655. (Biology). View Reference
Linfor J, Berger T. Potential role of alphav and beta1 integrins as oocyte adhesion molecules during fertilization in pigs. J Reprod Fertil. 2000; 120(1):65-72. (Biology). View Reference
Richard C, Thibaudeau K, Charreau B, et al. Characterization of a murine monoclonal antibody specific for swine beta1 integrin. Xenotransplantation. 1998; 5(1):75-83. (Immunogen: ELISA, Immunoaffinity chromatography, Immunocytochemistry (cytospins), Immunofluorescence, Immunoprecipitation, Western blot). View Reference
Springer TA. Adhesion receptors of the immune system. Nature. 1990; 346(6283):425-434. (Biology). View Reference

View All (4)

561496 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.