Multicolor flow cytometric analysis of CD3 expression on pig peripheral blood lymphocytes. Pig whole blood was stained simultaneously with Alexa Fluor® 647 Mouse Anti-Pig CD3ε antibody (Cat. No. 561476) and PE-Cy™7 Mouse Anti-Pig CD4 antibody (Cat. No. 561473). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). A two-color flow cytometric dot plot showing the correlated expression of CD3 versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Multicolor flow cytometric analysis of CD3 expression on pig peripheral blood lymphocytes. Pig whole blood was stained simultaneously with Alexa Fluor® 647 Mouse Anti-Pig CD3ε antibody (Cat. No. 561476) and PE-Cy™7 Mouse Anti-Pig CD4 antibody (Cat. No. 561473). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). A two-color flow cytometric dot plot showing the correlated expression of CD3 versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Pharmingen™
CD3 epsilon subunit; CD3e; T-cell surface glycoprotein CD3 epsilon chain
Pig (QC Testing)
Mouse BALB/c IgG2a, κ
Pig peripheral blood mononuclear cells
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_10683311
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control RUO
Size
100 Tests
Cat No.
557715
PE-Cy™7 Mouse Anti-Pig CD4a RUO
Size
50 µg
Cat No.
561473
561476 Rev. 1
Antibody Details
BB23-8E6-8C8
The BB23-8E6-8C8 monoclonal antibody specifically binds to the 25-kDa ε chain of the T-cell receptor-associated CD3 complex. It recognizes all CD4+ and most CD8+ peripheral blood T lymphocytes, most thymocytes and phytohemagglutinin-stimulated blasts, and subsets of spleen and Peyer's patch lymphocytes. BB23-8E6-8C8 is a immunoglobulin isotype switch variant of the BB23-8E6 clone. This isotype-switch variant induces a proliferative response of peripheral blood mononuclear cells. The epitope recognized by BB23-8E6 mAb was designated CD3a by the Second International Swine CD Workshop.
561476 Rev. 1
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
561476 Rev.1
Citations & References
View product citations for antibody "561476" on CiteAb
Development References (2)
-
Pescovitz MD, Book BK, Aasted B. Analyses of monoclonal antibodies reacting with porcine CD3: results from the Second International Swine CD Workshop. Vet Immunol Immunopathol. 1998; 60(3-4):261-268. (Clone-specific). View Reference
-
Pescovitz MD, Book BK, Aasted B. Summary of workshop findings for antibodies reacting with porcine T-cells and activation antigens: results from the Second International Swine CD Workshop. Vet Immunol Immunopathol. 1998; 60(3-4):251-260. (Clone-specific). View Reference
561476 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.