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      FITC Mouse Anti-Human CD39
      FITC Mouse Anti-Human CD39
      Flow cytometric analysis of CD39 expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stained simultaneously with Alexa Fluor® 700 Anti-Human CD4 (Cat. No. 557922/561030), APC Mouse Anti-Human CD25 ( Cat. No. 555434/560987), and PE Mouse Anti-Human CD127 ( Cat No. 557938/561028) antibodies and with either FITC Mouse Anti-Human CD39 antibody (Cat. No. 561444) or a FITC Mouse IgG2b, κ Isotype Control (Cat. No. 555742). Regulatory T cells were identified from the gated events based on their light scattering characteristics as lymphocytes and fluorescence characteristics of CD4+ cells shown as a CD25 bright and CD127 dim population (Left Panel). CD39 expression is shown on regulatory T cells (Right Panel, solid line histogram) versus Ig Isotype Control staining (Right Panel; dotted line histogram). Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
      FITC Mouse Anti-Human CD39
      Flow cytometric analysis of CD39 expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stained simultaneously with Alexa Fluor® 700 Anti-Human CD4 (Cat. No. 557922/561030), APC Mouse Anti-Human CD25 ( Cat. No. 555434/560987), and PE Mouse Anti-Human CD127 ( Cat No. 557938/561028) antibodies and with either FITC Mouse Anti-Human CD39 antibody (Cat. No. 561444) or a FITC Mouse IgG2b, κ Isotype Control (Cat. No. 555742). Regulatory T cells were identified from the gated events based on their light scattering characteristics as lymphocytes and fluorescence characteristics of CD4+ cells shown as a CD25 bright and CD127 dim population (Left Panel). CD39 expression is shown on regulatory T cells (Right Panel, solid line histogram) versus Ig Isotype Control staining (Right Panel; dotted line histogram). Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
      Product Details
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      BD Pharmingen™
      ENTPD1; NTPDase-1; Ecto-ATPase 1; Ecto-ATPDase 1
      Human (QC Testing)
      Mouse IgG2b, κ
      Flow cytometry (Routinely Tested)
      5 µl
      IV A54
      953
      AB_10896292
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
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      Product Notices

      1. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      2. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      3. An isotype control should be used at the same concentration as the antibody of interest.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
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      561444 Rev. 1
      Antibody Details
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      TU66

      The TU66 monoclonal antibody specifically recognizes human CD39 which is also known as Ectonucleoside triphosphate diphosphohydrolase 1 (NTPDase 1), Ecto-ATP diphosphohydrolase 1 (Ecto-ATPDase 1), or Ecto-apyrase. CD39 is an integral membrane glycoprotein with two transmembrane domains, N- and C-terminal cytoplasmic tails, and an extracellular region that contains the NTPDase 1 active site. CD39 is encoded by ENTPD1 which belongs to the ectoenzyme family. CD39 is variably expressed on activated T cells and B cells, regulatory T cells (Treg), dendritic cells, Langerhans cells, NK cells, monocytes, macrophages, endothelial cells, and granulocytes. CD39 acts on extracellular nucleoside triphosphates and diphosphates including ATP and ADP that are hydrolyzed into AMP. Through cell surface CD73 (Ecto-5'-nucleotidase), regulatory T cells can act on extracellular AMP to generate immunosuppressive adenosine. CD39 is involved in the control of the extracellular pool of phosphorylated nucleosides, the suppression of inflammation and immunity, and the regulation of platelet activation.

      561444 Rev. 1
      Format Details
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      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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      FITC
      Blue 488 nm
      494 nm
      518 nm
      561444 Rev.1
      Citations & References
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      View product citations for antibody "561444" on CiteAb

      Development References (4)

      1. Borsellino G, Kleinewietfeld M, Di Mitri D, et al. Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression.. Blood. 2007. (Biology). View Reference
      2. Duensing S, Kirshner H, Atzpodien J. CD39 as a novel marker of in vivo immune activation. Blood. 1994; 83(12):3826-3827. (Biology). View Reference
      3. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
      4. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
      View All (4) View Less
      561444 Rev. 1

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.