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      Alexa Fluor® 700 Rat Anti-Mouse Ly-6G
      Alexa Fluor® 700 Rat Anti-Mouse Ly-6G
      Alexa Fluor® 700 Rat Anti-Mouse Ly-6G
      Flow cytometric analysis of Ly-6G expression on mouse bone-marrow leukocytes. A BALB/c bone-marrow cell suspension was stained with either Alexa Fluor® 700 Rat anti-Mouse Ly-6G antibody (Cat. No. 561236; solid line histogram) or Alexa Fluor® 700 rat IgG2a, κ Isotype Control (Cat. No. 557963; dashed line histogram).  Flow cytometric histograms were derived from gated events based on the light scattering characteristics of viable lymphoid (Left Panel) or myeloid (Right Panel) cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.
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      561236
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      $182.00
      EA (1 Each)
      50 µg
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      Product Details
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      BD Pharmingen™
      Ly6g; Lymphocyte antigen 6G; Lymphocyte antigen 6 complex, locus G; Gr1
      Mouse (QC Testing)
      Rat LEW, also known as Lewis IgG2a, κ
      Ly-6G-transfected EL4J Cell Line
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      AB_10611860
      Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 700 under optimum conditions, and unreacted Alexa Fluor® 700 was removed.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
      5. Alexa Fluor® 700 has an adsorption maximum of ~700nm and a peak fluorescence emission of ~720nm. Before staining cells with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
      6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      Data Sheets

      DownloadTechnical Data Sheet (TDS)DownloadSafety Data Sheet
      561236 Rev. 2
      Antibody Details
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      1A8

      The 1A8 monoclonal antibody specifically binds to Ly-6G, a 21-25-kDa GPI-anchored protein. In the bone marrow, Ly6G is expressed on the majority of the largest cells, predominantly granulocytes, but not on lymphoid or erythroid cells.  In the periphery, it is expressed on granulocytes. The mAb RB6-8C5 recognizes both Ly-6G and Ly-6C and blocks the binding of mAb 1A8 to Ly-6G.

      561236 Rev. 2
      Format Details
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      Alexa Fluor™ 700
      Alexa Fluor™ 700 dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 697 nm and an emission maximum (Em Max) at 719-nm. Alexa Fluor™ 700 is designed to be excited by the Red (627–640-nm) laser and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 700
      Red 627-640 nm
      697 nm
      719 nm
      561236 Rev.2
      Citations & References
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      Development References (2)

      1. Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 1993; 151(5):2399-2408. (Immunogen). View Reference
      2. Fleming TJ, Malek TR. Multiple glycosylphosphatidylinositol-anchored Ly-6 molecules and transmembrane Ly-6E mediate inhibition of IL-2 production. J Immunol. 1994; 153(5):1955-1962. (Biology). View Reference
      561236 Rev. 2

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.