Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
The MAR1-5A3 monoclonal antibody specifically binds to the IFN-α/β Receptor 1 (also known as Interferon-alpha/beta Receptor 1; Type I Interferon Receptor 1 or IFNAR-1) subunit. A variety of cell types including lymphocytes, monocytes/macrophages, dendritic cells, and fibroblasts can be stimulated or induced by viral and microbial infections to produce and secrete type I interferons, including Interferon-alpha (IFN-α) subtypes and Interferon-beta (IFN-β). IFN-α subtypes and IFN-β bind to a common heterodimeric receptor complex (also known as the Type I IFN Receptor or IFN-α/β Receptor) that is comprised of transmembrane glycoprotein IFNAR-1 and IFNAR-2 subunits and is expressed on most cell types. Upon ligand binding, IFNAR-1 and IFNAR-2 signal cellular responses. Type I interferons are multifunctional proteins that can induce antiviral states in cells as well as regulate the activation, growth, proliferation, differentiation and viability of various cell types including cells that mediate innate and adaptive immunity as well as autoimmune diseases. The MAR1-5A3 monoclonal antibody blocks Type I IFN receptor signaling and in vitro and in vivo biologic responses caused by type I interferons. The MAR1-5A3 antibody has also been reported to be useful for immunofluorescent staining and flow cytometric analysis, immunoprecipitation and Western blot analysis of IFNAR-1.
Development References (6)
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Dunn GP, Bruce AT, Sheehan KC, et al. A critical function for type I interferons in cancer immunoediting. Nat Immunol. 2005; 6(7):722-729. (Clone-specific: Blocking, Functional assay). View Reference
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Sheehan KC, Lai KS, Dunn GP, et al. Blocking monoclonal antibodies specific for mouse IFN-alpha/beta receptor subunit 1 (IFNAR-1) from mice immunized by in vivo hydrodynamic transfection. J Interferon Cytokine Res. 2006; 26(11):804-819. (Clone-specific: Blocking, ELISA, Flow cytometry, Functional assay). View Reference
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Strobl B, Bubic I, Bruns U, et al. Novel functions of tyrosine kinase 2 in the antiviral defense against murine cytomegalovirus. J Immunol. 2005; 175(6):4000-4008. (Biology). View Reference
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Swann JB, Hayakawa Y, Zerafa N, et al. Type I IFN contributes to NK cell homeostasis, activation, and antitumor function. J Immunol. 2007; 178(2):7540-7549. (Clone-specific: Blocking, Functional assay). View Reference
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Theofilopoulos AN, Baccala R, Beutler B, Kono DH. Type I interferons (alpha/beta) in immunity and autoimmunity. Annu Rev Immunol. 2005; 23:307-336. (Biology). View Reference
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Uze G, Lutfalla G, Bandu MT, Proudhon D, Mogensen KE. Behavior of a cloned murine interferon alpha/beta receptor expressed in homospecific or heterospecific background. Proc Natl Acad Sci U S A. 1992; 89(10):4774-4778. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.