Skip to main content Skip to navigation
Hamburger Menu
Search icon
Order lookup icon Order lookup icon
Order Lookup
Country Selector Country Selector
United States (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      APC Rat Anti-Mouse IgD
      APC Rat Anti-Mouse IgD
      Flow cytometric analysis of surface IgD on BALB/c mouse splenocytes. BALB/c splenocytes were simultaneously stained with APC Rat anti-Mouse IgD (Cat. No. 560868) and Alexa Fluor® 488 Hamster anti-Mouse CD3 (Cat. No. 557666). A two-color flow cytometric dot plots showing the correlated expression of IgD versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a BD LSR™ II Flow Cytometry System.
      APC Rat Anti-Mouse IgD
      Flow cytometric analysis of surface IgD on BALB/c mouse splenocytes. BALB/c splenocytes were simultaneously stained with APC Rat anti-Mouse IgD (Cat. No. 560868) and Alexa Fluor® 488 Hamster anti-Mouse CD3 (Cat. No. 557666). A two-color flow cytometric dot plots showing the correlated expression of IgD versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a BD LSR™ II Flow Cytometry System.
      Product Details
      Down Arrow Up Arrow


      BD Pharmingen™
      IGHD; Igh-5; Immunoglobulin heavy chain 5; Ig delta chain C region
      Mouse (QC Testing)
      Rat IgG2a, κ
      Not reported
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      380797
      AB_10612002
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      Show More blue down arrow Show Less blue up arrow
      560868 Rev. 1
      Antibody Details
      Down Arrow Up Arrow
      11-26c.2a

      The 11-26c.2a monoclonal antibody specifically binds to mouse immunoglobulin D of all Igh-C haplotypes (e.g., IgDa, IgDb, IgDe), and it does not react with other immunoglobulin isotypes.  Although 11-26c.2a mAb binds membrane IgD expressed on the splenic B-cell surface with high affinity, it does not induce proliferation of splenic B cells in vitro.  In vivo injection of 11-26c.2a antibody does not have any effect on activation of mature B cells, as determined by MHC class II antigen expression.

      560868 Rev. 1
      Format Details
      Down Arrow Up Arrow
      APC
      Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      APC
      Red 627-640 nm
      651 nm
      660 nm
      560868 Rev.1
      Citations & References
      Down Arrow Up Arrow
      View product citations for antibody "560868" on CiteAb

      Development References (3)

      1. Hamilton AM, Lehuen A, Kearney JF. Immunofluorescence analysis of B-1 cell ontogeny in the mouse. Int Immunol. 1994; 6(3):355-361. (Biology). View Reference
      2. Ishihara K, Wood WJ Jr, Wall R, et al. Multiple B29 containing complexes on murine B lymphocytes. Common and stage-restricted Ig-associated polypeptide chains. J Immunol. 1993; 150(6):2253-2262. (Biology). View Reference
      3. Nitschke L, Kosco MH, Kohler G, Lamers MC. Immunoglobulin D-deficient mice can mount normal immune responses to thymus-independent and -dependent antigens. Proc Natl Acad Sci U S A. 1993; 90(5):1887-1891. (Clone-specific: Flow cytometry). View Reference
      560868 Rev. 1

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.