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Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
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The 1D4B antibody recognizes CD107a which is also known as, Lysosome-Associated Membrane Protein 1 (LAMP-1). CD107a is one of the two major glycoproteins in lysosome membranes that provide useful markers to distinguish lysosomes from other organelles. CD107a may play a role in the lysosomal degradation of certain molecules. Mouse CD107a is a type I transmembrane glycoprotein. It consists of a 40-kDa core protein which is heavily glycosylated to form heterogeneous mature glycoprotein of 110-140 kDa. It is principally expressed in epithelial cells and macrophages in a variety of organs. Following activation, CD107a is relocated to the surface of some lymphocytes, macrophages, epithelial cells, endothelial cells, platelets, and tumor cells. Cell-surface CD107a may participate in intercellular adhesion and adhesion to the extracellular matrix. Cell surface CD107a expression can serve as a useful marker for cytotoxic NK and CD8+ T cells, as well as, some malignant tumor cells.
Development References (5)
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Arterburn LM, Earles BJ, August JT. The disulfide structure of mouse lysosome-associated membrane protein 1. J Biol Chem. 1990; 265(13):7419-7423. (Biology). View Reference
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Chen JW, Chen GL, D'Souza MP, Murphy TL, August JT. Lysosomal membrane glycoproteins: properties of LAMP-1 and LAMP-2. Biochem Soc Symp. 1986; 51:97-112. (Biology). View Reference
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Chen JW, Murphy TL, Willingham MC, Pastan I, August JT. Identification of two lysosomal membrane glycoproteins. J Cell Biol. 1985; 101(1):85-95. (Biology). View Reference
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Chen JW, Pan W, D'Souza MP, August JT. Lysosome-associated membrane proteins: characterization of LAMP-1 of macrophage P388 and mouse embryo 3T3 cultured cells. Arch Biochem Biophys. 1985; 239(2):574-586. (Immunogen). View Reference
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Rohrer J, Schweizer A, Russell D, Kornfeld S. The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane. J Cell Biol. 1996; 132(4):565-576. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.