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      PE Mouse Anti-Human CD328
      PE Mouse Anti-Human CD328
      Flow cytometric analysis of CD328 (Siglect-7) expression on peripheral blood monocytes. Peripheral blood monocytes were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 555749, dashed line histogram) or PE Mouse Anti-Human CD328 (Cat. No. 558372; solid line histogram). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monoocytes.
      PE Mouse Anti-Human CD328
      Flow cytometric analysis of CD328 (Siglect-7) expression on peripheral blood monocytes. Peripheral blood monocytes were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 555749, dashed line histogram) or PE Mouse Anti-Human CD328 (Cat. No. 558372; solid line histogram). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monoocytes.
      Product Details
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      BD Pharmingen™
      Siglec-7; Siglec7; AIRM1; sialic acid binding Ig like lectin 7
      Human (QC Testing)
      Mouse IgG1, κ
      Recombinant Siglec-7
      Flow cytometry (Routinely Tested)
      20 µl
      VIII 80435; IX 28
      AB_647230
      Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      558372 Rev. 4
      Antibody Details
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      F023-420

      The F023-420 monoclonal antibody specifically recognizes CD328 which is also known as sialic acid binding Ig like lectin 7 (Siglec-7). CD238 is an I-type lectin of approximately 65 kDa, expressed as a monomer on a major subset of NK cells and a subset of CD8+ cells. It is also expressed at moderate levels on monocytes and weakly on granulocytes. Siglecs (sialic acid/immunoglobulin/lectin) are a family of I-type lectins that bind to sialic acids on the cell surface. They are a family of carbohydrate binding proteins within the immunoglobulin superfamily. Siglecs are integral membrane proteins with extracellular N-terminal, V-set Ig domains, followed by variable numbers of C2-set Ig domains.

      558372 Rev. 4
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      558372 Rev.4
      Citations & References
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      View product citations for antibody "558372" on CiteAb

      Development References (4)

      1. Angata T, Hingorani R, Varki NM, Varki A. Cloning and characterization of a novel mouse Siglec, mSiglec-F: differential evolution of the mouse and human (CD33) Siglec-3-related gene clusters. J Biol Chem. 2001; 276(48):45128-45136. (Biology). View Reference
      2. Crocker PR, Varki A. Siglecs, sialic acids and innate immunity. Trends Immunol. 2001; 22(6):337-342. (Biology). View Reference
      3. Nicoll G, Ni J, Liu D, et al. Identification and characterization of a novel siglec, siglec-7, expressed by human natural killer cells and monocytes. J Biol Chem. 1999; 274(48):34089-34095. (Biology). View Reference
      4. Powell LD, Varki A. I-type lectins. J Biol Chem. 1995; 270(24):14243-14246. (Biology). View Reference
      View All (4) View Less
      558372 Rev. 4

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.