BD Pharmingen™ Purified Mouse Anti-Stat2 (pY690)

Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors.  Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation.  Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes.  Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner.  Stat1 and Stat2 are components of the ISGF3 (Interferon-Stimulated Gene Factor 3) complex, which is the primary transcription activator induced by the binding of the interferon to a specific cell-surface receptor.  Stat2 is a 113-kDa protein having approximately 40% homology with Stat1α.  Stat2 interacts with Stat1 for the efficient activation of Stat1 in response to IFN-α, which is essential for normal transcriptional activity of the ISGF3 complex.  Stat2 phosphorylation at the tyrosine 690 residue (Y690) after IFN-α activation can occur independently of Stat1, but the localization and nuclear stability of phosphorylated Stat2 is dependent on Stat1.

The 7a/Stat2 monoclonal antibody recognizes the phosphorylated Y690 of human Stat2.