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      APC Mouse Anti-Human CD314 (NKG2D)
      APC Mouse Anti-Human CD314 (NKG2D)
      Flow cytometric analysis of CD314 (NKG2D) expression on human peripheral blood lymphocytes. Whole blood was stained with APC Mouse anti-Human CD314 (NKG2D)(Cat. No. 558071/562064) and PE Mouse Anti-Human CD56 (Cat. No. 556647). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The two color dot plot was derived from gated events with the side and forward light-scatter characteristics of viable lymphocytes.
      APC Mouse Anti-Human CD314 (NKG2D)
      Flow cytometric analysis of CD314 (NKG2D) expression on human peripheral blood lymphocytes. Whole blood was stained with APC Mouse anti-Human CD314 (NKG2D)(Cat. No. 558071/562064) and PE Mouse Anti-Human CD56 (Cat. No. 556647). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The two color dot plot was derived from gated events with the side and forward light-scatter characteristics of viable lymphocytes.
      Product Details
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      BD Pharmingen™
      CD314; KLRK1; KLR; NKG2D; NKG2-D; NK cell receptor D
      Human (QC Testing)
      Mouse RBF/ DnJ IgG1, κ
      NKL cells
      Flow cytometry (Routinely Tested)
      20 µl
      VIII 80432
      22914
      AB_398654
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      558071 Rev. 5
      Antibody Details
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      1D11

      The 1D11 monoclonal antibody specifically binds to NKG2D, a 42 kDa type II transmembrane glycoprotein that is also known as CD314 and KLRK1. NKG2D is a member of the C-type lectin family and is expressed on human NK cells. This activating receptor binds strongly to several ligands including MICA and MICB and ULBP-1, -2, and -3 proteins that are expressed by different target cell types. Different from natural cytotoxicity receptor (NCR), NKG2D expression is not confined to NK cells. It is also expressed on virtually all TCR γ/δ+ and CD8+TCR α/β+ T cells. NKG2D functions as a triggering receptor involved in natural cytotoxicity mediated by normal NK cells against a variety of tumors or normal target cells. Importantly, NKG2D can complement the role of NCR in tumor cell lysis. Remarkably, the combined maskings of NCR and NKG2D can reportedly lead to a complete inhibition of NK-mediated lysis of all tumor or normal cells. The 1D11 antibody can reportedly block or stimulate the function of NKG2D-positive cells.

      558071 Rev. 5
      Format Details
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      APC
      Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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      APC
      Red 627-640 nm
      651 nm
      660 nm
      558071 Rev.5
      Citations & References
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      View product citations for antibody "558071" on CiteAb

      Development References (2)

      1. Bauer S, Groh V, Wu J, et al. Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science. 1999; 285(5428):727-729. (Biology). View Reference
      2. Groh V, Rhinehart R, Randolph-Habecker J, Topp MS, Riddell SR, Spies T. Costimulation of CD8alphabeta T cells by NKG2D via engagement by MIC induced on virus-infected cells. Nat Immunol. 2001; 2(3):255-260. (Biology). View Reference
      558071 Rev. 5

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.