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      FITC Mouse Anti-Human CD3ε
      FITC Mouse Anti-Human CD3ε
      Flow cytometric analysis of CD3ε expression on Rhesus monkey peripheral blood lymphocytes. Rhesus whole blood was stained with either FITC Mouse IgG1, κ Isotype Control (Cat. No. 556658; dashed line histogram) or FITC Mouse Anti-Human CD3 antibody  (Cat. No. 556611, solid line histogram). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
      FITC Mouse Anti-Human CD3ε
      Flow cytometric analysis of CD3ε expression on Rhesus monkey peripheral blood lymphocytes. Rhesus whole blood was stained with either FITC Mouse IgG1, κ Isotype Control (Cat. No. 556658; dashed line histogram) or FITC Mouse Anti-Human CD3 antibody  (Cat. No. 556611, solid line histogram). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
      Product Details
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      BD Pharmingen™
      Rhesus, Cynomolgus, Baboon (QC Testing), Human (Tested in Development)
      Mouse BALB/c IgG3, λ
      Human CD3 Proteins
      Flow cytometry (Routinely Tested)
      20 µl
      V 5T-CD03.05
      AB_396484
      Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      556611 Rev. 8
      Antibody Details
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      SP34

      Clone SP34 reacts with human form of the 20 kDa epsilon subunit of the CD3 antigen/T-cell receptor (TCR) complex. This clone also cross-reacts with a major subset of peripheral blood lymphocytes, but not monocytes or granulocytes, of rhesus, cynomolgus, and pigtail macaque monkeys. The distribution on lymphocytes is similar to that observed with normal human donor lymphocytes, with the majority of CD3-positive cells being negative when dual stained with antibodies to B or NK cell markers.

      556611 Rev. 8
      Format Details
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      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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      FITC
      Blue 488 nm
      494 nm
      518 nm
      556611 Rev.8
      Citations & References
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      View product citations for antibody "556611" on CiteAb

      Development References (2)

      1. Sancho J, Ledbetter JA, Choi MS, Kanner SB, Deans JP, Terhorst C. CD3-zeta surface expression is required for CD4-p56lck-mediated upregulation of T cell antigen receptor-CD3 signaling in T cells. J Biol Chem. 1992; 267(11):7871-7879. (Biology). View Reference
      2. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
      556611 Rev. 8

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.