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      Biotin Mouse Anti-Rat IL-4
      Product Details
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      BD Pharmingen™
      Rat (QC Testing)
      Mouse IgG1, κ
      Immunoaffinity-purified, recombinant rat IL-4
      ELISA Detection (Routinely Tested)
      0.5 mg/ml
      AB_395692
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      ELISA: This biotinylated mouse anti-rat IL-4 antibody can be used as the detection antibody in a sandwich ELISA for measuring rat IL-4 protein levels in conjunction with purified OX-81 antibody (Cat. No. 555080) as the capture antibody and recombinant rat IL-4 protein (Cat. No. 555107) as the standard. This antibody pair does not cross react with rat IL-2, IL-10, TNF, GM-CSF, mouse IL-4, TNF, IFN-γ, human IL-4, IL-6, IL-10, IL-15, RANTES. This biotinylated detection antibody should be pretitrated in the range of 0.5 to 2 µg/ml to determine its optimal concentration for ELISA. To obtain linear standard curves, doubling dilutions of recombinant rat IL-4 protein (e.g., ranging from ~5,120 to 5 pg/ml) are recommended for inclusion in each ELISA plate. For specific methodology, please visit our web site, www.bdbiosciences.com, and go to the protocols section or the chapter on ELISA in the Immune Function Handbook.

      Note:  This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum or plasma samples. For measuring rat IL-4 in serum or plasma our rat IL-4 BD OptEIA set (Cat. No. 555198) is specially formulated and recommended.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      555090 Rev. 1
      Antibody Details
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      B11-3

      The B11-3 antibody reacts with rat interleukin-4 (IL-4). The immunogen used to generate the B11-3 hybridoma was immunoaffinity-purified, recombinant rat IL-4. This is a non-neutralizing antibody.

      This antibody is routinely tested by ELISA detection. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

      555090 Rev. 1
      Format Details
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      Biotin
      Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
      Biotin
      555090 Rev.1
      Citations & References
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      View product citations for antibody "555090" on CiteAb

      Development References (3)

      1. Prigent P, Saoudi A, Pannetier C, et al. Mercuric chloride, a chemical responsible for T helper cell (Th)2-mediated autoimmunity in brown Norway rats, directly triggers T cells to produce interleukin-4. J Clin Invest. 1995; 96(3):1484-1489. (Clone-specific: ELISA). View Reference
      2. Ramirez F, Fowell DJ, Puklavec M, Simmonds S, Mason D. Glucocorticoids promote a TH2 cytokine response by CD4+ T cells in vitro. J Immunol. 1996; 156(7):2406-2412. (Clone-specific: ELISA). View Reference
      3. Saoudi A, Simmonds S, Huitinga I, Mason D. Prevention of experimental allergic encephalomyelitis in rats by targeting autoantigen to B cells: evidence that the protective mechanism depends on changes in the cytokine response and migratory properties of the autoantigen-specific T cells. J Exp Med. 1995; 182(2):335-344. (Clone-specific: ELISA). View Reference
      555090 Rev. 1

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.