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      Biotin Mouse Anti-Rat CD90/Mouse CD90.1
      Biotin Mouse Anti-Rat CD90/Mouse CD90.1
      Comparison of the expression of CD90/CD90.1 by rat and mouse peripheral lymphocytes. Lewis rat splenocytes were simultaneously stained with PE-conjugated anti-rat CD3 G4.18 (Cat. No. 554833) and biotin-conjugated HIS51 monoclonal antibodies (left panel). P/J mouse splenocytes were  simultaneously stained with PE-conjugated anti-mouse CD3e 145-2C11 (Cat. No.  553063/553064) and biotin-conjugated HIS51 monoclonal antibodies (right panel). The second-step reagent was Avidin-FITC (Cat. No. 554057, both panels). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
      Biotin Mouse Anti-Rat CD90/Mouse CD90.1
      Comparison of the expression of CD90/CD90.1 by rat and mouse peripheral lymphocytes. Lewis rat splenocytes were simultaneously stained with PE-conjugated anti-rat CD3 G4.18 (Cat. No. 554833) and biotin-conjugated HIS51 monoclonal antibodies (left panel). P/J mouse splenocytes were  simultaneously stained with PE-conjugated anti-mouse CD3e 145-2C11 (Cat. No.  553063/553064) and biotin-conjugated HIS51 monoclonal antibodies (right panel). The second-step reagent was Avidin-FITC (Cat. No. 554057, both panels). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
      Product Details
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      BD Pharmingen™
      Thy-1/Thy-1.1
      Rat (QC Testing), Mouse (Tested in Development)
      Mouse BALB/c IgG2a, κ
      Rat bone marrow cells
      Flow cytometry (Routinely Tested)
      0.5 mg/ml
      24832,21838
      AB_395584
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      554893 Rev. 11
      Antibody Details
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      HIS51

      The HIS51 monoclonal antibody specifically binds to the rat Thy-1 antigen (CD90) expressed by hematopoietic stem cells, early myeloid and erythroid cells, immature B lymphocytes in the bone marrow and peripheral lymphoid organs, thymocytes, recent thymic emigrants (a subset of CD45RC- peripheral T cells), neurons, glomerular mesangial cells, endothelium at inflammatory sites, mast cells, and bone marrow-derived dendritic cells. Rat dendritic epidermal T cells (DEC) are Thy-1-negative. CD90 is a GPI-anchored membrane glycoprotein of the Ig superfamily which is involved in signal transduction. In addition, there is evidence in the mouse that CD90 mediates adhesion of thymocytes to thymic stroma. HIS51 antibody cross-reacts with the mouse Thy-1.1 alloantigen of the AKR/J and PL strains, but not Thy-1.2 found on most strains. In the mouse, CD90 is found on thymocytes, most peripheral T lymphocytes, some intraepithelial T lymphocytes (IEL, DEC), hematopoietic stem cells, and neurons, but not B lymphocytes.

      554893 Rev. 11
      Format Details
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      Biotin
      Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
      Biotin
      554893 Rev.11
      Citations & References
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      View product citations for antibody "554893" on CiteAb

      Development References (12)

      1. Campbell DG, Gagnon J, Reid KB, Williams AF. Rat brain Thy-1 glycoprotein. The amino acid sequence, disulphide bonds and an unusual hydrophobic region. Biochem J. 1981; 195(1):15-30. (Biology). View Reference
      2. Chen-Woan M, Delaney CP, Fournier V, et al. In vitro characterization of rat bone marrow-derived dendritic cells and their precursors. J Leukoc Biol. 1996; 59(2):196-207. (Biology). View Reference
      3. Dráberová L, Amoui M, and Dráber P. Thy-1-mediated activation of rat mast cells: the role of Thy-1 membrane microdomains. Immunology. 1996; 87(1):141-148. (Biology). View Reference
      4. Elbe A, Kilgus O, Hünig T, and Stingl G. T-cell receptor diversity in dendritic epidermal T cells in the rat. J Invest Dermatol. 1993; 102:74-79. (Biology). View Reference
      5. Groen H, Klatter FA, van Petersen AS, Pater JM, Nieuwenhuis P, Kampinga J. Composition of rat CD4+ resting memory T-cell pool is influenced by major histocompatibility complex. Transplant Proc. 1993; 25(5):2782-2783. (Biology). View Reference
      6. He HT, Naquet P, Caillol D, Pierres M. Thy-1 supports adhesion of mouse thymocytes to thymic epithelial cells through a Ca2(+)-independent mechanism. J Exp Med. 1991; 173(2):515-518. (Biology). View Reference
      7. Hermans MH, Opstelten D. In situ visualization of hemopoietic cell subsets and stromal elements in rat and mouse bone marrow by immunostaining of frozen sections. J Histochem Cytochem. 1991; 39(12):1627-1634. (Immunogen). View Reference
      8. Hosseinzadeh H, Goldschneider I. Recent thymic emigrants in the rat express a unique antigenic phenotype and undergo post-thymic maturation in peripheral lymphoid tissues. J Immunol. 1993; 150(5):1670-1679. (Biology). View Reference
      9. Ishizu A, Ishikura H, Nakamaru Y et al. Thy-1 induced on rat endothelium regulates vascular permeability at sites of inflammation. Int Immunol. 1995; 7:1939-1947. (Biology). View Reference
      10. Kroese FG, de Boer NK, de Boer T, Nieuwenhuis P, Kantor AB, Deenen GJ. Identification and kinetics of two recently bone marrow-derived B cell populations in peripheral lymphoid tissues. Cell Immunol. 1995; 162(2):185-193. (Biology). View Reference
      11. Nakashima I, Pu M-Y, Hamaguchi M, et al. Pathway of signal delivery to murine thymocytes triggered by co-crosslinking CD3 and Thy-1 for cellular DNA fragmentation and growth inhibition. J Immunol. 1993; 151(7):3511-3520. (Biology).
      12. Paul LC, Rennke HG, Milford EL, and Carpenter CB. Thy-1.1 in glomeruli of rat kidneys. Kidney Int. 1984; 25:771-777. (Biology). View Reference
      View All (12) View Less
      554893 Rev. 11

       

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      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.