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Product Details
BD Pharmingen™
H-2kd; H2kd; MHC class I H-2Kd
Mouse (QC Testing)
Mouse SJL IgG2a, κ
BALB/c Mouse Cells
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_394924
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
PE Mouse IgG2a, κ Isotype Control RUO
Size
0.1 mg
Cat No.
553457
Purified Hamster Anti-Mouse CD3e RUO
Size
0.1 mg
Cat No.
557306
Purified Hamster Anti-Mouse CD3e RUO
Size
0.5 mg
Cat No.
553058
PE Mouse Anti-Mouse H-2K[d] RUO
Size
25 µg
Cat No.
562004
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553566 Rev. 18
Antibody Details
SF1-1.1
The SF1-1.1 antibody reacts with the α3 domain of the H-2K[d] MHC class I alloantigen. Reactivity with other haplotypes (e.g, b, j, k, p, q, s, v) has not been observed. It has been reported that plate-bound SF1-1.1 mAb moderately enhances the apoptotic response of thymocytes to plate-bound 145-2C11 mAb (anti-mouse CD3e).
553566 Rev. 18
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 561 nm
496 nm, 566 nm
576 nm
553566 Rev.18
Citations & References
View product citations for antibody "553566" on CiteAb
Development References (3)
-
Abastado JP, Casrouge A, Kourilsky P. Differential role of conserved and polymorphic residues of the binding groove of MHC class I molecules in the selection of peptides. J Immunol. 1993; 151(7):3569-3575. (Clone-specific). View Reference
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Noun G, Reboul M, Abastado JP, Jaulin C, Kourilsky P, Pla M. Alloreactive monoclonal antibodies select Kd molecules with different peptide profiles. J Immunol. 1996; 157(6):2455-2461. (Clone-specific). View Reference
-
Zhao Y, Iwata M. Cross-linking of the TCR-CD3 complex with CD4, CD8 or LFA-1 induces an anti-apoptotic signal in thymocytes: the signal is canceled by FK506. Int Immunol. 1995; 7(9):1387-1396. (Biology). View Reference
553566 Rev. 18
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.