Skip to main content Skip to navigation
Hamburger Menu
Search icon
Mini Cart
Empty CartYou are not signed in.
Order lookup icon Order lookup icon
Order Lookup
Country Selector Country Selector
United States (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Mini Cart
Empty CartYou are not signed in.
Products

Link Arrow
Solutions

Link Arrow
Discover & Learn

Link Arrow
Resources & Tools

Link Arrow
Support

Link Arrow
Search icon Search icon
Close Menu
    Mini Cart
    Empty CartYou are not signed in.
    Expand menu Expand menu
      Loading...
      Alert Icon
      Purified Rat Anti-Mouse CD102
      552854
      Sign In
      $246.00
      EA (1 Each)
      0.5 mg
      Add to Cart IconAdd to Cart
      Save To Shopping ListRequest Bulk Reagent Quote
      Product Details
      Down Arrow


      BD Pharmingen™
      ICAM-2
      Mouse (QC Testing)
      Rat LEW, also known as Lewis IgG2a, κ
      Transfected Cell Line
      Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Blocking, Immunoprecipitation (Reported), Immunohistochemistry-paraffin (Not Recommended)
      0.5 mg/ml
      15896
      AB_394784
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
      Show More blue down arrow Show Less blue up arrow

      Data Sheets

      DownloadTechnical Data Sheet (TDS)DownloadSafety Data Sheet
      553326 Rev. 11
      Antibody Details
      Down Arrow
      3C4(mIC2/4)

      The 3C4 (mIC2/4) monoclonal antibody specifically binds to the mouse ICAM-2 (CD102) cell surface glycoprotein, a ligand for LFA-1. CD102 is constitutively expressed on endothelial cells, T and B lymphocytes, and alveolar walls. It is also expressed on a variety of leukocyte cell lines. CD102 does not appear to be involved in the development of hematopoietic cells. In a model for allergic asthma, endothelial CD102 mediates the transmigration of eosinophils (but not lymphocytes, monocytes, or macrophages) into the airway lumen. The 3C4 (mIC2/4) antibody blocks interactions between ICAM-2 and LFA-1.

      This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

      553326 Rev. 11
      Format Details
      Down Arrow
      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      553326 Rev.11
      Citations & References
      Down Arrow

      Development References (3)

      1. Gerwin N, Gonzalo JA, Lloyd C, et al. Prolonged eosinophil accumulation in allergic lung interstitium of ICAM-2 deficient mice results in extended hyperresponsiveness. Immunity. 1999; 10(1):9-19. (Clone-specific: Immunohistochemistry). View Reference
      2. Xu H, Bickford JK, Luther E, Carpenito C, Takei F, Springer TA. Characterization of murine intercellular adhesion molecule-2. J Immunol. 1996; 156(12):4909-4914. (Immunogen: Blocking, Immunoprecipitation). View Reference
      3. Xu H, Tong IL, De Fougerolles AR, Springer TA. Isolation, characterization, and expression of mouse ICAM-2 complementary and genomic DNA. J Immunol. 1999; 149(8):2650-2655. (Biology). View Reference
      553326 Rev. 11

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.